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Sample GSM281548 Query DataSets for GSM281548
Status Public on Apr 24, 2008
Title Ozone_1h
Sample type RNA
 
Channel 1
Source name Ozone_Control_Replication1
Organism Oryza sativa
Characteristics Healthy plants, untreated
Treatment protocol Healthy seedlings at 0 h, control
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy3
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
Channel 2
Source name Ozone_Treated_Replication1
Organism Oryza sativa
Characteristics Fumigated plants
Treatment protocol Fumigated for 1 h
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy5
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
 
Hybridization protocol Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies
Scan protocol Standard protocol of Gene Pix Scanner
Description Keyword = rice
Keyword = ozone
Data processing The GeneSpring Ver. 4 software was used for normalization.
 
Submission date Apr 13, 2008
Last update date Apr 23, 2008
Contact name Randeep Rakwal
E-mail(s) rakwal-68@aist.go.jp
Fax +81 29 861 6066
Organization name AIST
Lab MST, HTRC lab
Street address AIST WEST, Onagawa 16-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8569
Country Japan
 
Platform ID GPL892
Series (1)
GSE11157 Microarray analysis of rice plants fumigated with ozone

Data table header descriptions
ID_REF
VALUE Log10 ( Norm_CH2 / Norm_CH1)
Norm_CH1 Normalised Intensity of Raw_CH1
Norm_CH2 Normalised Intensity of Raw_CH2
RAW_CH1 Background subtracted Intensity of CH1
RAW_CH2 Background subtracted Intensity of CH1

Data table
ID_REF VALUE Norm_CH1 Norm_CH2 RAW_CH1 RAW_CH2
1 -0.408203084 471 184 434 48
2 -0.991226076 441 45 425 45
3 -0.301029996 30 15 27 15
4 0.102662342 60 76 60 74
5 -0.007424018 59 58 60 61
6 -0.916453949 33 4 34 2
7 -0.272258253 73 39 54 25
8 0.055222791 59 67 59 66
9 -0.640978057 35 8 32 7
10 -0.189056236 34 22 31 21
11 0.085283845 106 129 107 132
12 0.025028006 118 125 119 121
13 -0.107905397 50 39 49 38
14 -0.050046039 147 131 153 132
15 0.057991947 70 80 70 80
16 -0.397940009 25 10 24 10
17 -0.016599262 80 77 80 77
18 0.057352809 85 97 87 99
19 -0.356547324 25 11 25 11
20 -0.110071459 67 52 67 51

Total number of rows: 22575

Table truncated, full table size 676 Kbytes.




Supplementary file Size Download File type/resource
GSM281548.gpr.gz 1.3 Mb (ftp)(http) GPR
Processed data included within Sample table

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