|
| Status |
Public on Apr 24, 2008 |
| Title |
Ozone_12h |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Ozone_Control_Replication1
|
| Organism |
Oryza sativa |
| Characteristics |
Healthy plants, untreated
|
| Treatment protocol |
Healthy seedlings at 0 h, control
|
| Growth protocol |
Plants were grown in growth chamber in soil for 14 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL Reagent method
|
| Label |
Cy3
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
Ozone_Treated_Replication1
|
| Organism |
Oryza sativa |
| Characteristics |
Fumigated plants
|
| Treatment protocol |
Fumigated for 12 h
|
| Growth protocol |
Plants were grown in growth chamber in soil for 14 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL Reagent method
|
| Label |
Cy5
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies
|
| Scan protocol |
Standard protocol of Gene Pix Scanner
|
| Description |
Keyword = rice
Keyword = ozone
|
| Data processing |
The GeneSpring Ver. 4 software was used for normalization.
|
| |
|
| Submission date |
Apr 13, 2008 |
| Last update date |
Apr 23, 2008 |
| Contact name |
Randeep Rakwal |
| E-mail(s) |
rakwal-68@aist.go.jp
|
| Fax |
+81 29 861 6066
|
| Organization name |
AIST
|
| Lab |
MST, HTRC lab
|
| Street address |
AIST WEST, Onagawa 16-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8569 |
| Country |
Japan |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE11157 |
Microarray analysis of rice plants fumigated with ozone |
|
