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Sample GSM281555 Query DataSets for GSM281555
Status Public on Apr 24, 2008
Title Ozone_24h
Sample type RNA
 
Channel 1
Source name Ozone_Control_Replication1
Organism Oryza sativa
Characteristics Healthy plants, untreated
Treatment protocol Healthy seedlings at 0 h, control
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy3
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
Channel 2
Source name Ozone_Treated_Replication1
Organism Oryza sativa
Characteristics Fumigated plants
Treatment protocol Fumigated for 12 h
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy5
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
 
Hybridization protocol Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies
Scan protocol Standard protocol of Gene Pix Scanner
Description Keyword = rice
Keyword = ozone
Data processing The GeneSpring Ver. 4 software was used for normalization.
 
Submission date Apr 13, 2008
Last update date Apr 23, 2008
Contact name Randeep Rakwal
E-mail(s) rakwal-68@aist.go.jp
Fax +81 29 861 6066
Organization name AIST
Lab MST, HTRC lab
Street address AIST WEST, Onagawa 16-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8569
Country Japan
 
Platform ID GPL892
Series (1)
GSE11157 Microarray analysis of rice plants fumigated with ozone

Data table header descriptions
ID_REF
VALUE Log10 ( Norm_CH2 / Norm_CH1)
Norm_CH1 Normalised Intensity of Raw_CH1
Norm_CH2 Normalised Intensity of Raw_CH2
RAW_CH1 Background subtracted Intensity of CH1
RAW_CH2 Background subtracted Intensity of CH1

Data table
ID_REF VALUE Norm_CH1 Norm_CH2 RAW_CH1 RAW_CH2
1 -0.729459326 236 44 243 39
2 -0.767234836 275 47 269 41
3 -1.041392685 22 2 22 2
4 -0.034762106 52 48 51 44
5 -0.061053965 61 53 61 51
6 -0.430124692 35 13 34 13
7 -0.496006599 47 15 48 12
8 0.435229093 58 158 57 157
9 -1.146128036 28 2 28 2
10 -0.397940009 30 12 30 11
11 -0.580661847 99 26 99 26
12 0.189537983 106 164 108 163
13 0.009984221 43 44 42 44
14 -0.199829106 141 89 140 93
15 -0.025554104 70 66 71 65
16 -0.698970004 20 4 20 3
17 0.100281009 77 97 76 96
18 -0.070037867 94 80 99 77
19 -0.14976232 24 17 25 16
20 -0.061656901 68 59 69 59

Total number of rows: 22575

Table truncated, full table size 676 Kbytes.




Supplementary file Size Download File type/resource
GSM281555.gpr.gz 1.3 Mb (ftp)(http) GPR
Processed data included within Sample table

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