|
| Status |
Public on Dec 14, 2017 |
| Title |
F3 |
| Sample type |
RNA |
| |
|
| Source name |
Human liver biopsies
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
gender: Male
age: 58
disease state: NAFLD
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and RNA integrity was determined by 1% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
The lncRNAs were labeled using the Agilent human lncRNA+mRNA Array V4.0. Total RNA (200 ng) was dephosphorylated and ligated with pCp-Cy3, the labeled RNA was purified and hybridized to lncRNA arrays.
|
| |
|
| Hybridization protocol |
According to the Agilent lncRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
After hybridization and washes, slides were scanned immediately in Agilent Microarray Scanner with Surescan High Resolution Technology (Agilent Technologies, Santa Clara, CA).
|
| Description |
Human liver biopsies from 5 patients with NAFLD and 5 healthy control subjects were performed with patient consent within the diagnostic workup of NAFLD
|
| Data processing |
Feature Extraction v10.10 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and Bioconductor software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Nov 21, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Jian Li |
| E-mail(s) |
lijian@bjhmoh.cn
|
| Organization name |
Beijing Hospital
|
| Street address |
Dahua Road
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE107231 |
lncRNA changes in the livers of NAFLD patients compared with that of healthy control |
|
