|
| Status |
Public on Feb 12, 2024 |
| Title |
Non-tumor fetal CD140a+ CTX, sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
Dissociated cells from normal fetal CTX, sorted on CD140a
|
| Organism |
Homo sapiens |
| Characteristics |
tumor type: non-tumor (normal)
tumor grade: non-tumor (normal)
cell sort marker: CD140a+ (PDGFRa)
developmental stage: fetal, 20.5wk
tissue source: cortex
|
| Treatment protocol |
Tissue samples were dissociated with papain, cultured in suspension plates in serum-free media, and sorted MACS and FACS on A2B5 (tumor, adult normal samples) or CD140a (fetal normal samples).
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNA was extracted and purified with the Qiagen miRNeasy kit, according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufacturer's instructions.
|
| Scan protocol |
GeneChip scanning and image processing were performed according to the manufacturer's instructions.
|
| Description |
CD140a_FETAL_CTX_NONTUMOR_2
Small RNA
miRNA expression from Dissociated cells from normal fetal CTX, sorted on CD140a.
|
| Data processing |
Raw intensity values were normalized using Variance Stabilizing Normalization (VSN) with RMA-style probeset summarization, as implemented by the vsnrma function of the VSN package in Bioconductor, in the R statistical software environment. As the Affymetrix miRNA 3.0 array platform includes miRNA for multiple species, prior to differential expression analysis data was filtered to include probes corresponding to mature human miRNAs.
|
| |
|
| Submission date |
Jan 02, 2018 |
| Last update date |
Feb 12, 2024 |
| Contact name |
Steven Goldman |
| Organization name |
University of Rochester Medical Center
|
| Department |
Center for Translational Neuromedicine
|
| Lab |
Goldman Lab
|
| Street address |
601 Elmwood Ave
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE255589 |
Human Adult Glial Progenitors Express Distinct miRNA Profiles Compared to their Fetal Counterparts |
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