|
| Status |
Public on Aug 23, 2018 |
| Title |
MDA-ER-beta E2 #3 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-ER-beta E2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231-ER-beta
cell type: Triple Negative Breast Cancer Cell Line
treated with: 17-beta estradiol for 5 days
|
| Treatment protocol |
MDA-MB-231-ER-beta cells were plated in 10 cm dishes and allowed to grow to approximately 80% confluence in DMEM/F12 medium containing 10% charcoal stripped FBS and Doxycycline (100 ng/ml, to induce ER-beta expression). Cells were subsequently treated with ethanol vehicle or 1 nM estradiol for 5 days. Media was changed on day 3 to refresh treatments.
|
| Growth protocol |
Doxycycline-inducible ER-beta expressing MDA-MB-231cells were maintained in phenol red-free DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS), 1% antibiotic-antimycotic (AA), 5 mg/L blasticidin S and 500 mg/L zeocin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using Trizol per the manufacturer's recommendation.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
9271668028_B
|
| Data processing |
The data were normalized using Faster cyclic loess (Fastlo) in R
|
| |
|
| Submission date |
Jan 09, 2018 |
| Last update date |
Aug 24, 2018 |
| Contact name |
Jason Carroll |
| E-mail(s) |
Jason.Carroll@cruk.cam.ac.uk
|
| Phone |
+44 1223 769649
|
| Organization name |
Cancer Research UK, Cambridge Institute
|
| Street address |
Li Ka Shing Centre, Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 ORE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10904 |
| Series (2) |
| GSE108980 |
Identification of the ERβ transcriptome in MDA-MB-231 cells [microarray] |
| GSE108981 |
Identification of the ERβ transcriptome in ER-beta expressing MDA-MB-231 cells |
|
