|
| Status |
Public on Aug 26, 2009 |
| Title |
Human.AN.CP.ECRB |
| Sample type |
RNA |
| |
|
| Source name |
Extensor carpi radialis brevis muscle from cerebral palsy patient
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
Age: 12
SL: 4.56
House: 3
Severity: SS
Ashworth: 3
Zancolli: 2B
PEFF: 50
PESF: 45
AEFF: -90
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Kit (Qaigen) extraction of total RNA was performed
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16-18 hr at 45C on GeneChip Human Genome U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
gene expression data from cerebral palsy muscle
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS5) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. The data were also analyzed with Robust Multiarray Analysis (RMA) and GC Robust Multiarray Analysis (GCRMA) using GeneSpring GX 7.3 (Agilent Technologies). The RMA and GCRMA data are available as supplementary files to the Series record.
|
| |
|
| Submission date |
Jun 04, 2008 |
| Last update date |
Aug 26, 2009 |
| Contact name |
Lucas Robert Smith |
| E-mail(s) |
lrs001@ucsd.edu
|
| Organization name |
University of California San Diego
|
| Department |
Bioengineering
|
| Lab |
Muscle Physiology Laboratory
|
| Street address |
3350 La Jolla Village Drive Mail Code 151
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92161 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE11686 |
Unique Transcriptional Profile in Wrist Muscles From Cerebral Palsy Patients |
|
