|
| Status |
Public on Dec 01, 2019 |
| Title |
healthy group sample3F |
| Sample type |
RNA |
| |
|
| Source name |
Homo sapiens
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
Sex: female
group: healthy
age: 20y
|
| Treatment protocol |
Fresh whole blood from healthy volunteers and patients with post hepatitis B infection liver cirrhosis was collected and stored in BD PAXgeneTM whole blood RNA tubes and mixed upside down，- 20 ℃ to -80 ℃ cryopreserved
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
control group
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Dec 17, 2018 |
| Last update date |
Dec 01, 2019 |
| Contact name |
leijia Li |
| E-mail(s) |
lovelylileijia@163.com
|
| Organization name |
The third affiliated hospital of sun yat-sen university
|
| Street address |
Tianhe road
|
| City |
guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE123932 |
Development of gene expression signatures for liver cirrhosis |
|
