|
| Status |
Public on Jan 29, 2009 |
| Title |
JAY270xS288c CGH-array |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
JAY270 genomic DNA labeled with Cy5
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
S. cerevisiae strain JAY270 total genomic DNA.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA prepared using standard yeast DNA extraction protocol.
|
| Label |
Cy5
|
| Label protocol |
Direct incorporation with klenow polymerase and random priming.
|
| |
|
| Channel 2 |
| Source name |
S288c genomic DNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
S. cerevisiae strain FY86 (S288c) total genomic DNA.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA prepared using standard yeast DNA extraction protocol.
|
| Label |
Cy3
|
| Label protocol |
Direct incorporation with klenow polymerase and random priming.
|
| |
|
| |
| Hybridization protocol |
Described in Argueso et al PNAS (2008) 105:11845
|
| Scan protocol |
Array scanned in Genepix 4000b scanner.
|
| Description |
This is a Comparative Genome Hybridization between the bioethanol diploid strain JAY270 and the reference laboratory strain FY86 which is isogenic to S288c. Note that the arrays used were designed based on the S288c genome.
|
| Data processing |
The log2 R/G ratio for all probes in the arrays were collapsed to a single value in the case of repeated probes and normalized according to the median value for the entire array.
|
| |
|
| Submission date |
Jan 27, 2009 |
| Last update date |
Jan 28, 2009 |
| Contact name |
Juan Lucas Argueso |
| E-mail(s) |
lucas.argueso@duke.edu
|
| Phone |
1-919-684-5814
|
| Organization name |
Duke University Medical Center
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
371 CARL Building, Box 3054
|
| City |
Durham |
| State/province |
SP |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL4414 |
| Series (1) |
| GSE14601 |
Comparative Genomic Hybridization between S. cerevisaie strains JAY270 and S288c. |
|
