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Status |
Public on Jan 29, 2009 |
Title |
Comparative Genomic Hybridization between S. cerevisaie strains JAY270 and S288c. |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Genome variation profiling by array
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Summary |
The arrays in this series correspond to a comparative genomic analysis between S. cerevisiae strain JAY270 and the reference laboratory strain S288c. JAY270 is a heterothalic diploid used in bioethanol production from sugar cane feedstock in Brazil. This strain has several chromosomal length polymorphisms between homologous chromosomes. The two Chr6 homologs, Chr6 short and Chr6 long, were examined using microarrays to determine the genomic regions which are rearranged.
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Overall design |
Three arrays are provided in this series. In all three total genomic DNA from a S288c isogenic strain was labeled with Cy3 and provides a reference for competitive hybridization to the arrays. The first array in the series is a full CGH-array comparing the complete JAY270 diploid genome to S288c. The second and third arrays correspond the PFGE-purified Chr6 short and Chr6 long DNA from JAY270 compared to the S288c genome (Band-arrays). The arrays used in this study were designed using the genome sequence of the reference S288c strain.
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Contributor(s) |
Argueso JL |
Citation(s) |
19812109 |
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Submission date |
Jan 27, 2009 |
Last update date |
Mar 20, 2012 |
Contact name |
Juan Lucas Argueso |
E-mail(s) |
lucas.argueso@duke.edu
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Phone |
1-919-684-5814
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Organization name |
Duke University Medical Center
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Department |
Molecular Genetics and Microbiology
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Street address |
371 CARL Building, Box 3054
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City |
Durham |
State/province |
SP |
ZIP/Postal code |
27710 |
Country |
USA |
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Platforms (1) |
GPL4414 |
Lieb Lab at UNC-CH_Yeast Whole-genome Array (PCR-based)_for Buck MJ et al. |
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Samples (7)
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Relations |
BioProject |
PRJNA111721 |