|
| Status |
Public on Jan 29, 2009 |
| Title |
JAY270 Chr6 long band array |
| Sample type |
mixed |
| |
|
| Channel 1 |
| Source name |
JAY270 Chr6 long DNA labeled with Cy5
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
PFGE-purified Chrmosomal DNA from strain JAY270 Chr6 long.
|
| Extracted molecule |
other |
| Extraction protocol |
DNA specific to JAY270 Chr6-short, which was separated in Pulse-Field gel electrophoresis and labeled with Cy5
|
| Label |
Cy5
|
| Label protocol |
Direct incorporation with klenow polymerase and random priming.
|
| |
|
| Channel 2 |
| Source name |
S288c genomic DNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Total genomic DNA from S288c isogenic strain.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA prepared using standard yeast DNA extraction protocol.
|
| Label |
Cy3
|
| Label protocol |
Direct incorporation with klenow polymerase and random priming.
|
| |
|
| |
| Hybridization protocol |
Described in Argueso et al PNAS (2008) 105:11845
|
| Scan protocol |
Array scanned in Genepix 4000b scanner.
|
| Description |
This is a Band-array experiment in which Cy5-labeled DNA from JAY270 Chr6 long was mixed to Cy3-labeled total genomic S288c DNA and hybridized to microarrays containing probes for the entire S288c genome. This experiment allows the determination of chromosomal rearrangements between the JAY270 and S288c yeast strain backgrounds.
|
| Data processing |
The log2 R/G ratio for all probes in the arrays were collapsed to a single value in the case of repeated probes and normalized according to the median value for the entire array.
|
| |
|
| Submission date |
Jan 27, 2009 |
| Last update date |
Jan 28, 2009 |
| Contact name |
Juan Lucas Argueso |
| E-mail(s) |
lucas.argueso@duke.edu
|
| Phone |
1-919-684-5814
|
| Organization name |
Duke University Medical Center
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
371 CARL Building, Box 3054
|
| City |
Durham |
| State/province |
SP |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL4414 |
| Series (1) |
| GSE14601 |
Comparative Genomic Hybridization between S. cerevisaie strains JAY270 and S288c. |
|
