|
| Status |
Public on Feb 14, 2009 |
| Title |
RD MD-E, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
RD cells infected with retrovirus expressing MyoD~E heterodimers
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RD cells
infection: retrovirus expressing MyoD~E heterodimers
|
| Treatment protocol |
RD cells were retrovirally infected with either MyoD~E heterodimer or an empty control vector, selected for 48 hours in the presence of 1 ug/mL puromycin, and then placed in DMEM media supplemented with 1%Horse serum, Insulin and Transferrin for 24 hours.
|
| Growth protocol |
RD cells were grown in DMEM media supplemented with 10% Bovine Calf Serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared using Qiagen RNeasy Mini Kit according to the manufacture's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HGU133 plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from RD cells infected with retroviral vector expressing MyoD~E heterodimers
|
| Data processing |
The data were normalized by RMA using the Affy package of Bioconductor
|
| |
|
| Submission date |
Feb 13, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yi Cao |
| E-mail(s) |
ycao@fhcrc.org
|
| Phone |
206-667-5278
|
| Organization name |
Fred Hutchinson CRC
|
| Department |
Human Biology
|
| Lab |
Tapscott
|
| Street address |
1100 Fairview Ave. N
|
| City |
Seatttle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14825 |
Expression data from rhabdomyosarcoma cells expressing Myod and E-protein heterodimer and controls |
|
| Relations |
| Reanalyzed by |
GSE119087 |
