|
Status |
Public on Sep 14, 2009 |
Title |
NSG200 |
Sample type |
RNA |
|
|
Source name |
whole blood from rheumatoid arthritis patient treated with anti_TNF therapy
|
Organism |
Homo sapiens |
Characteristics |
tissue: whole blood disease state: rheumatoid arthritis protocol: anti_TNF therapy disease activity (das28_v1): 6.14 disease activity (das28_v3): 2.15 response to anti-tnf therapy: RESPONSE
|
Biomaterial provider |
ABCON [Autoimmune Biomarkers Collaborative Network]
|
Treatment protocol |
anti-TNF therapy
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from PaxGene tubes as per the manufacturer’s standard protocol.
|
Label |
biotin
|
Label protocol |
RNA was amplified, fragmented and labeled using FL Ovation cDNA biotin automated module V2 (cat #A4200, Nugen inc., San Carlos CA ) and FL Ovation cDNA biotin automated module V2 (cat #A4200, Nugen inc., San Carlos CA ) using Beckman Arrayplex automated workstation according to manufactures protocol
|
|
|
Hybridization protocol |
3 ug of fragmented and labeled cDNA was hybridized HGU-133 plus 2 arrays
|
Scan protocol |
Genechip cartridge arrays using the Genechip Array Station (Affymetrix, Santa Clara, CA).
|
Description |
no additional information
|
Data processing |
Data was normalized with GCRMA and the proccessed using limma Bioconductor package
|
|
|
Submission date |
Mar 17, 2009 |
Last update date |
Sep 14, 2009 |
Contact name |
Jadwiga Bienkowska |
E-mail(s) |
jbienkowska@gmail.com
|
Phone |
6174602469
|
Organization name |
BiogenIdec
|
Street address |
45 Fayette Str Apt 3
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02139 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE15258 |
Whole blood transcript profiling of rheumatoid arthritis patients |
|