|
| Status |
Public on Nov 04, 2019 |
| Title |
Si29_3_post: Patient 9 after splenectomy-2 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
surgical indication: immune thrombocytopenic purpura
total/partial resection: total
age: 30
Sex: male
surgical approach: laparoscopic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Total RNA Purification Kit from Norgen Biotek corp following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Labbeling was done according to Nimblegen one color DNA Labeling kit.
|
| |
|
| Hybridization protocol |
Hybridization done according to Agilent Gene expression hybridization kit
|
| Scan protocol |
Scanned on Agilent SureScan Microarray Scanner (Agilent Technologies G2600D SG12434244)
|
| Description |
Si29_3_post
|
| Data processing |
Data pre-processing steps of background-correction, normalization and summarization were performed in R using functions in Limma library. The raw intensity for each probe is the median feature pixel intensity with the median background subtracted. Data was quantile normalized followed by log2 transform. Signals from probes measuring same ncRNA were averaged.
|
| |
|
| Submission date |
Jul 01, 2019 |
| Last update date |
Nov 04, 2019 |
| Contact name |
George Calin |
| E-mail(s) |
gcalin@mdanderson.org
|
| Phone |
713-792-5461
|
| Organization name |
MD Anderson Cancer Center
|
| Department |
Experimental Therapeutics
|
| Street address |
1881 East Road
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL22858 |
| Series (1) |
| GSE133589 |
The non-coding RNome after splenectomy (cohort B) |
|
