|
| Status |
Public on Apr 14, 2021 |
| Title |
Gastric cancer and adjacent noncancerous tissues from patient 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Gastric cancer tissue of patient3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Cancer tissue of patient3
age: 68
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent(Invitrogen,Camarillo,USA) following the manufacturer's instructions and purified with NucleoSpin? RNA clean-up (Macherey-nagel,Duren,Germany,Cat. No.740609.250). RNA purity and concentration were determined from OD260/280 readings using spectrophotometer (NanoDrop ND-1000) and RNA integrity was determined by 1.2% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy5 and Cy3-dCTP) was produced by Eberwine¡¯s linear RNA amplification method and subsequent enzymatic reaction.
|
| |
|
| Channel 2 |
| Source name |
Adjacent noncancerous tissues
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Paired adjacent noncancerous tissue of patient3
age: 68
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent(Invitrogen,Camarillo,USA) following the manufacturer's instructions and purified with NucleoSpin? RNA clean-up (Macherey-nagel,Duren,Germany,Cat. No.740609.250). RNA purity and concentration were determined from OD260/280 readings using spectrophotometer (NanoDrop ND-1000) and RNA integrity was determined by 1.2% formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy5 and Cy3-dCTP) was produced by Eberwine¡¯s linear RNA amplification method and subsequent enzymatic reaction.
|
| |
|
| |
| Hybridization protocol |
The labeled RNA was purified and hybridized to microarrays according to the Agilent manufacturer's instructions.
|
| Scan protocol |
Agilent chip scanner (G2565CA) was used to scan the cleaned chip and obtain the hybrid picture
|
| Description |
Gastric tissue and paired adjacent noncancerous tissue from patient3
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 01, 2020 |
| Last update date |
Apr 14, 2021 |
| Contact name |
Min Deng |
| E-mail(s) |
mindeng@gzhmu.edu.cn
|
| Phone |
86-20-66673666
|
| Organization name |
Affiliated Cancer Hospital & Institute of Guangzhou Medical University
|
| Street address |
78 Hengzhigang Road
|
| City |
Guangzhou |
| ZIP/Postal code |
510095 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE157289 |
LncRNA expression profile in gastric cancer specimens and adjacent noncancerous tissues |
|
