|
| Status |
Public on Jan 07, 2022 |
| Title |
Balanced constitution_PBMCs Replicate 7 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Balanced constitution_ peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMCs
age: 51
female: female
diagnosis: Balanced constitution
|
| Growth protocol |
The isolated PBMCs were treated with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to avoid RNA degradation, and then stored at -80°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
14 µl cDNA ,obtained after reverse transcription and purification, were primed with 4 µl Random primer at 95°C for 3 min, then reversed transcribed at 37°C for 1.5 h in the presence of Nucleospin® Extract II (MN company,Cat. No. 740609.250), and the purified product was labeled with 1 tube cy3-dCTP or another tube Cy5-dCTP.After the labeled and purified products were mixed, a total of 60 µl was vacuumized and concentrated to 27.5 µl for later use.
|
| |
|
| Channel 2 |
| Source name |
pooled reference
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMCs
|
| Growth protocol |
The isolated PBMCs were treated with TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to avoid RNA degradation, and then stored at -80°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
14 µl cDNA ,obtained after reverse transcription and purification, were primed with 4 µl Random primer at 95°C for 3 min, then reversed transcribed at 37°C for 1.5 h in the presence of Nucleospin® Extract II (MN company,Cat. No. 740609.250), and the purified product was labeled with 1 tube cy3-dCTP or another tube Cy5-dCTP.After the labeled and purified products were mixed, a total of 60 µl was vacuumized and concentrated to 27.5 µl for later use.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Crystal Core ® biochip Universal labeling kit) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
Agilent Feature Extraction(V10.7) software was used to analyze and extract data from scanned images.
|
| Description |
Biological replicate 7 of 9.Balanced constitution, peripheral blood mononuclear cells.
|
| Data processing |
Agilent GeneSpring(V13.0) software was used for data normalization and difference analysis.
|
| |
|
| Submission date |
Sep 24, 2020 |
| Last update date |
Jan 07, 2022 |
| Contact name |
Lidan Dong |
| E-mail(s) |
2018011004@bucm.edu.cn
|
| Phone |
13167022696
|
| Organization name |
北京中医药大学
|
| Lab |
National Institute of TCM Constitution and Preventive Medicine
|
| Street address |
北京北三环东路11号
|
| City |
北京 |
| State/province |
北京 |
| ZIP/Postal code |
100000 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE158042 |
Humans PBMCs: Balanced constitution vs. Phlegm-dampness constitution |
|
