Targets made by synthesis of cDNA from total RNA (no amplification step).
K562 erythroleukemia cells (American Type Culture Collection CCL-243) were cultured in RPMI-1640 medium with L-glutamine supplemented with 10% fetal bovine serum, 100 IU/ml penicillin, and 100 microg/ml streptomycin. Cells were maintained at 37 deg C under 5% CO2/95% air in a humidified incubator.
Reference purchased from Stratagene. RNA pooled from 10 human cell lines: Adenocarcinoma, mammary gland Hepatoblastoma, liver Adenocarcinoma, cervix Embryonal carcinoma, testis Glioblastoma, brain Melanoma Liposarcoma Histiocytic lymphoma; macrophase; histocyte Lymphoblastic leukemia Plasmacytoma; myeloma; B lymphocyte
Measure of signal strength, calculated as 0.5 log2 (K562 sample intensity) + 0.5 log2 (reference sample intensity). Signal intensities were corrected by subtraction of background intensity from foreground intensity.
VALUE
Measure of differential gene expression, calculated as log2 ((K562 sample intensity)/(reference sample intensity)). Signal intensities were corrected by subtraction of background intensity from foreground intensity.
