|
| Status |
Public on Apr 16, 2021 |
| Title |
160726B_L3 |
| Sample type |
RNA |
| |
|
| Source name |
SCLC with BM
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: SCLC patient with BMs and 6
tissue: Peripheral blood
|
| Treatment protocol |
Human PBMCs were isolated with Ficoll-Hypaque gradients.
|
| Growth protocol |
Peripheral blood anticoagulated by ethylenediaminetetraacetic acid was acquired from all SCLC patients.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from PBMCs using Trizol® reagent (Invitrogen, Grand Island, NY, USA).
|
| Label |
Cy3
|
| Label protocol |
The total RNAs was labeled to lncRNA + mRNA Human Gene Expression Microarray V4.0 (4x180K), each array contained a probe set comprising 40,916 human lncRNA transcripts and 34,235 human mRNAs.
|
| |
|
| Hybridization protocol |
1 μg of total RNA was used for reverse transcription into cDNAs according to the manufacturer’s instructions. Then, the cDNA was labeled with Cy3-dCTP and hybridized to the Agilent Human CBC lncRNA + mRNA microarray V4.0
|
| Scan protocol |
The microarrays were washed and scanned using a G2505C Microarray Scanner (Agilent). Data were normalized and analyzed using GeneSpring software (Agilent).
|
| Description |
L3:gProcessedSignal(normalized)
|
| Data processing |
Data were normalized and analyzed using GeneSpring software (Agilent).
|
| |
|
| Submission date |
Nov 22, 2020 |
| Last update date |
Apr 16, 2021 |
| Contact name |
Yaru Tian |
| E-mail(s) |
1798378336@qq.com
|
| Organization name |
Shandong Cancer Hospital
|
| Street address |
Jiyan Road
|
| City |
Jinan |
| ZIP/Postal code |
250117 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE161968 |
Downregulation of lncRNA XR_429159.1 could promote brain metastasis in patients with limited-stage small-cell lung cancer |
|
