|
| Status |
Public on Dec 30, 2020 |
| Title |
PBMCs_T2D_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood mononuclear cells, T2D, replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
gender: male
age: 55y
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
Gene expression in T2D patient peripheral blood mononuclear cells
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Dec 29, 2020 |
| Last update date |
Dec 30, 2020 |
| Contact name |
Qi Ma |
| E-mail(s) |
maqi1212@126.com
|
| Phone |
(+86)15999172776
|
| Organization name |
First Affiliated Hospital of Xinjiang Medical University
|
| Street address |
No.91, Liyushan Road
|
| City |
Urumqi |
| ZIP/Postal code |
830054 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE163980 |
Long non-coding RNA screening for type 2 diabetes |
|
