|
| Status |
Public on Mar 20, 2021 |
| Title |
M85-Entorhinal Cortex (array) |
| Sample type |
genomic |
| |
|
| Source name |
Entorhinal Cortex
|
| Organism |
Mus musculus |
| Characteristics |
mouse_id: M85
sentrix_id: 204027420007_R03C02
Sex: F
group: 2
tissue: Entorhinal Cortex
|
| Treatment protocol |
Mice were terminally anaesthetized with pentobarbital (intraperitoneal injection) and transcardially perfused with phosphate-buffered saline (PBS). The entorhinal cortex was dissected from the left brain hemisphere on wet ice and snap-frozen on dry ice for subsequent analysis
|
| Growth protocol |
Animals were housed under standard conditions (constant temperature and humidity) with a 12h light/dark cycle in individually ventilated cages, with free access to food (Teklad irradiated global rodent diet (Envigo, United Kingdom)) and water.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA from all samples was isolated from the entorhinal cortex using the AllPrep DNA/RNA Mini Kit (Qiagen)
|
| Label |
cy3, cy5
|
| Label protocol |
DNA methylation was measured using the the Mammalian Methylation Array (HorvathMammalMethylChip4, GEO: GPL28271)
|
| |
|
| Hybridization protocol |
Standard Illumina protocols
|
| Scan protocol |
Illumina HiScan
|
| Data processing |
Normalized Average Beta calculated using the SeSame package in R
|
| |
|
| Submission date |
Mar 19, 2021 |
| Last update date |
Mar 20, 2021 |
| Contact name |
Emma Walker |
| E-mail(s) |
e.m.walker@exeter.ac.uk
|
| Organization name |
University of Exeter
|
| Street address |
RILD Barrack Rd
|
| City |
Exeter |
| ZIP/Postal code |
EX2 5DW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL28271 |
| Series (2) |
| GSE169218 |
Characterizing the properties of bisulfite sequencing data: maximizing power and sensitivity to identify differences in DNA methylation [Array] |
| GSE169235 |
Characterizing the properties of bisulfite sequencing data: maximizing power and sensitivity to identify differences in DNA methylation |
|
