|
| Status |
Public on Feb 23, 2022 |
| Title |
Fetal alcohol exposed, 2 years replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Chlorocebus aethiops hippocampus sacrificed at 2 years after prenatal alcohol exposure
|
| Organism |
Chlorocebus aethiops |
| Characteristics |
age: Sacrificed at 2 years
ethanol exposure: Pre Natal Alcohol Exposure
|
| Treatment protocol |
Whole hippocampus was extracted and placed in a 1 ml sterile vial containing GITC
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Qiazol extraction performed according to manufacturers specifications
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan at the McGill Genome Center
|
| Description |
mRNA expression data from the hippocampus of vervet green monkeys sacrificed at 2 years after birth
|
| Data processing |
The data were analyzed with FlexArray software and the Bioconductor package in R. These data were normalized using the Robust Multi-Array (RMA) normalization method. Probe sets have been trimmed for present/absent calls, annotation, and where multiple probe sets were available for a single gene only the probe set showing the highest mean expression value was selected
|
| |
|
| Submission date |
Apr 28, 2021 |
| Last update date |
Feb 24, 2022 |
| Contact name |
Roberta M Palmour |
| E-mail(s) |
roberta.palmour@mcgill.ca
|
| Phone |
5143987303
|
| Organization name |
McGill
|
| Department |
Psychiatry
|
| Street address |
1033 Pine West
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A1A3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE173516 |
mRNA expression analysis of the hippocampus in a vervet monkey model of fetal alcohol spectrum disorder |
|
