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| Status |
Public on May 02, 2024 |
| Title |
Sample-1: CX01_RNASeq, biological rep1 |
| Sample type |
SRA |
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| Source name |
P. Paox1-SKL, P. Pdas1-skl, P. pDAK, B. mMDH3-skl, B. mACT1-skl, P. pPYC, P. sXR, P. sXDH, P. sXK, PEX5, ∆PEX31, ∆PEX32, S.c MDH2, S. cMDH3, S. Cnde1
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| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: CX01
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| Growth protocol |
Shake flask 30 ˚C, 200rpm
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| Extracted molecule |
total RNA |
| Extraction protocol |
Send to GENEWIZ
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
Cells were incubate in YPD medium overninght, and then transfer into Delft medium for 16h, finally cells were transfer into Delft medium without glucose but with 1% methanol as sole carbon source. Cells were collected at OD600 around 1.0
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| Data processing |
The raw reads from each sample were mapped to the CEN.PK 113-7D reference genome (http://cenpk.tudelft.nl) using TopHat (v 2.0.12)
Cufflinks version 2.2.166 was used to calculate FPKM values, and the feature Counts module of the Subread package (v 1.4.6) was used to determine raw read counts.
Genome_build: CEN.PK 113-7D
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| Submission date |
May 04, 2021 |
| Last update date |
May 02, 2024 |
| Contact name |
chunjun zhan |
| E-mail(s) |
chunjunzhan@lbl.gov
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| Phone |
5715233586
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| Organization name |
JEBI
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| Street address |
2131 NAPA St
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| City |
Richmond |
| State/province |
CA |
| ZIP/Postal code |
94804 |
| Country |
USA |
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| Platform ID |
GPL21656 |
| Series (1) |
| GSE173802 |
Expression data from Saccharomyces cerevisiae |
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| Relations |
| BioSample |
SAMN19006206 |
| SRA |
SRX10767417 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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