|
| Status |
Public on Dec 31, 2010 |
| Title |
mDC hypoxia 2 |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte derived dendritic cells cultured under hypoxic condition
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Monocyte derived dendritic cells
treatment group: hypoxic condition
disease status: healthy donor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was independently isolated from DCs derived from the Mn of three different donors using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
The RNA was reverse-transcribed into cDNA and biotin labeled according to the Affymetrix’s instructions.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was used for hybridization to Affymetrix HG-U133 plus 2.0 arrays according to the Affymetrix’s instructions.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000.
|
| Description |
Human peripheral blood mononuclear cells (PBMC) were isolated from venous blood of voluntary healthy donors. All cultures were maintained in RPMI 1640 medium supplemented with 10% FBS heat inactivated, 50μg/ml gentamycin. Monocytes were purified by negative selection. To generate hypoxic mDC the monocytes were incubated in six-well culture plates for 4 days under hypoxic conditions.
|
| Data processing |
Expression values were quantified and array quality control was performed with the statistical algorithms implemented in Affymetrix Microarray Suite 5.0.
|
| |
|
| Submission date |
Jun 10, 2010 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Paolo Fardin |
| E-mail(s) |
paolofardin@ospedale-gaslini.ge.it
|
| Organization name |
Istiuto Giannina Gaslini
|
| Department |
Molecular Biology
|
| Street address |
Largo G. Gaslini 5
|
| City |
Genoa |
| ZIP/Postal code |
16147 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22282 |
Mature dendritic cells under hypoxic condition |
|
| Relations |
| Reanalyzed by |
GSE86362 |
