|
| Status |
Public on May 02, 2011 |
| Title |
Low-grade fibromyxoid sarcoma 5 |
| Sample type |
RNA |
| |
|
| Source name |
soft tissue
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: tumor biopsy
tissue: Low-grade fibromyxoid sarcoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the TRIzol reagent (Invitrogen) and purified with Qiagen´s RNeasy kit according to the manufacturers´ instructions.
|
| Label |
biotin
|
| Label protocol |
cDNA was labelled with TdT using the WT Terminal Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Biotinylated cDNA was hybridized to GeneChip Gene 1_0 st arrays. Arrays were washed and stained on an Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanning was carried out with the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Global gene expression data
|
| Data processing |
Image analysis was perfomed using the GeneChip Operating Software (GCOS, Affymetrix). Background correction, normalization and probe summarization was done with the robust multichip average (RMA) algorithm implemented in the Expression Console software (Affymetrix).
|
| |
|
| Submission date |
Sep 25, 2010 |
| Last update date |
Sep 11, 2017 |
| Contact name |
Emely Möller |
| E-mail(s) |
emely.moller@med.lu.se
|
| Organization name |
Lund University
|
| Department |
Clinical Genetics
|
| Street address |
Lund University Hospital
|
| City |
Lund |
| ZIP/Postal code |
SE-221 85 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE24369 |
Gene expression profiling of low-grade fibromyxoid sarcoma (LGFMS) |
|
| Relations |
| Reanalyzed by |
GSE35162 |
| Reanalyzed by |
GSE103677 |
