|
Status |
Public on Aug 22, 2012 |
Title |
Early stage ovarian carcinoma T104 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Tumor
|
Organism |
Homo sapiens |
Characteristics |
gender: Female tissue: ovarian carcinoma tissue sample: T104 age: 61 Stage: Ia type: C grade: 3 ct: yes status: Dead os (months): 13 progression: yes pfs (months): 10
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNAzol (Invitrogen) according to manufacturer's protocol
|
Label |
Cy3
|
Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
|
|
Channel 2 |
Source name |
Female reference DNA
|
Organism |
Homo sapiens |
Characteristics |
gender: Female tissue: blood pooled from 18 healthy individuals
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNAzol (Invitrogen) according to manufacturer's protocol
|
Label |
Cy5
|
Label protocol |
DNA labeling protocol according to Enzo Life Sciences
|
|
|
|
Hybridization protocol |
Hybridization protocol available via www.agilent.com
|
Scan protocol |
Slides were scanned using the Agilent microarray scanner, protocol according to Agilent.
|
Description |
reference DNA is pooled DNA from the blood of 18 healthy individuals raw data files: MAL_251901510436_S01_CGH-v4_95_Feb07_1_2.txt, MAL_251901510436_S01_CGH-v4_95_Feb07_1_2.txt
|
Data processing |
Dewaving: The data have been de-waved as described in Van de Wiel et al. (2009) using the R package NoWaves. This is available from www.few.vu.nl/~mavdwiel/nowaves.html. Agilent 105K normals were used as calibration. Normalization: mode normalization.
|
|
|
Submission date |
Sep 28, 2010 |
Last update date |
Aug 22, 2012 |
Contact name |
Daoud Sie |
E-mail(s) |
d.sie@vumc.nl
|
Phone |
+31 20 4442428
|
Organization name |
Vrije Universiteit Medical Center
|
Department |
Pathology
|
Lab |
Microarray Core Facility
|
Street address |
De Boelelaan 1117
|
City |
Amsterdam |
ZIP/Postal code |
1081 HV |
Country |
Netherlands |
|
|
Platform ID |
GPL8693 |
Series (1) |
GSE24418 |
Genetic Aberrations Relate Early and Advanced Stage Ovarian Cancer and are Associated with Survival |
|