|
Status |
Public on Nov 28, 2023 |
Title |
7DAP endosperm, replicate 2, scRNAseq |
Sample type |
SRA |
|
|
Source name |
Endosperm
|
Organism |
Zea mays |
Characteristics |
tissue: Endosperm cultivar: B73 age: 7 DAP genotype: wild type
|
Growth protocol |
B73 inbried line kernels cultivated in the greenhouse of China Agricultural University (Beijing, China)
|
Extracted molecule |
total RNA |
Extraction protocol |
Endosperm from maize 80-100 maize kernels from 6 to 8 DAP. Endosperm were incubated in a enzymatic solution to digest cell walls and produce protoplasts. Protoplasts were filtered using a cell strainer and used as input for the 10x Chromium Single Cell 3' V3 pipeline. Libraries were prepared using Chromium Single Cell 3' Reagent Kit V3.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
10x Genomics
|
Data processing |
Raw data were compared, barcoded and quantified by UMI using the Chromium 10x Cell Ranger software package. Samples were normalized and cell clusters were determined using Seurat. Assembly: Zea mays ssp mays B73 v4 Supplementary files format and content: Cell Ranger output files (barcodes.tsv, features.tsv, matrix.mtx)
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|
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Submission date |
Apr 26, 2022 |
Last update date |
Nov 28, 2023 |
Contact name |
Qiang Huo |
E-mail(s) |
huoqiang@cau.edu.cn
|
Organization name |
China Agricultural University
|
Street address |
Yuanmingyuan West Road
|
City |
Beijing |
ZIP/Postal code |
100091 |
Country |
China |
|
|
Platform ID |
GPL25410 |
Series (2) |
GSE201640 |
Decoding the gene regulatory network of endosperm differentiation in maize [scRNA-seq] |
GSE201701 |
Decoding the gene regulatory network of endosperm differentiation in maize |
|
Relations |
BioSample |
SAMN27861975 |
SRA |
SRX15010906 |