|
| Status |
Public on Nov 28, 2023 |
| Title |
7DAP endosperm, replicate 2, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
Endosperm
|
| Organism |
Zea mays |
| Characteristics |
tissue: Endosperm
cultivar: B73
age: 7 DAP
genotype: wild type
|
| Growth protocol |
B73 inbried line kernels cultivated in the greenhouse of China Agricultural University (Beijing, China)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Endosperm from maize 80-100 maize kernels from 6 to 8 DAP. Endosperm were incubated in a enzymatic solution to digest cell walls and produce protoplasts. Protoplasts were filtered using a cell strainer and used as input for the 10x Chromium Single Cell 3' V3 pipeline. Libraries were prepared using Chromium Single Cell 3' Reagent Kit V3.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
Raw data were compared, barcoded and quantified by UMI using the Chromium 10x Cell Ranger software package. Samples were normalized and cell clusters were determined using Seurat.
Assembly: Zea mays ssp mays B73 v4
Supplementary files format and content: Cell Ranger output files (barcodes.tsv, features.tsv, matrix.mtx)
|
| |
|
| Submission date |
Apr 26, 2022 |
| Last update date |
Nov 28, 2023 |
| Contact name |
Qiang Huo |
| E-mail(s) |
huoqiang@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Street address |
Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100091 |
| Country |
China |
| |
|
| Platform ID |
GPL25410 |
| Series (2) |
| GSE201640 |
Decoding the gene regulatory network of endosperm differentiation in maize [scRNA-seq] |
| GSE201701 |
Decoding the gene regulatory network of endosperm differentiation in maize |
|
| Relations |
| BioSample |
SAMN27861975 |
| SRA |
SRX15010906 |
