|
| Status |
Public on Nov 28, 2023 |
| Title |
B73 7DAP endosperm, replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Endosperm
|
| Organism |
Zea mays |
| Characteristics |
tissue: Endosperm
cultivar: B73
age: 7 DAP
genotype: wild type
|
| Growth protocol |
B73 inbried line kernels cultivated in the greenhouse of China Agricultural University (Beijing, China)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Twenty of 6, 7, 8 DAP endosperms of B73 were pooled for extracting total RNA with an RNAprep Pure Plant Kit (cat. no. DP441, TIANGEN)
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
7DAP2
Rowcount.txt
|
| Data processing |
Trimmomatic (version 0.39) was used to trim the sequence reads and STAR was used to align them to the B73 v4 reference genome. We computed gene expression levels for uniquely mapped reads by Using featureCounts (version 2.0.1).
Supplementary files format and content: Zea mays ssp mays B73 v4
Supplementary files format and content: Matrix table with raw counts for every sample (column) and gene (rows)
|
| |
|
| Submission date |
Apr 27, 2022 |
| Last update date |
Nov 28, 2023 |
| Contact name |
Qiang Huo |
| E-mail(s) |
huoqiang@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Street address |
Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100091 |
| Country |
China |
| |
|
| Platform ID |
GPL25410 |
| Series (2) |
| GSE201671 |
Decoding the gene regulatory network of endosperm differentiation in maize [RNA-seq] |
| GSE201701 |
Decoding the gene regulatory network of endosperm differentiation in maize |
|
| Relations |
| BioSample |
SAMN27908881 |
| SRA |
SRX15014383 |
