|
| Status |
Public on Oct 12, 2023 |
| Title |
HASMC,TGF-β,sample 2 |
| Sample type |
SRA |
| |
|
| Source name |
HASMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HASMC
cell type: Human Aortic Smooth Muscle Cells
genotype: WT
treatment: TGF-β,24h
|
| Treatment protocol |
Differentiation was induced by treatment of 2-day post HASMC cells with 10ug/ml TGF-β or 10ug/ml PDGF (Sigma),
|
| Growth protocol |
HASMC cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37℃
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miR-221 was transfected 24h after HASMC adherence and incubated for 48h
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
PromethION |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence sequence using porechop
Trimmed sequence reads were mapped to GRCh38 using minimap2
Transcript extraction and normalization were performed using FLAIR
Assembly: GRCh38
Supplementary files format and content: tab-delimited text files include TPM values for each Sample
|
| |
|
| Submission date |
Jul 25, 2022 |
| Last update date |
Oct 12, 2023 |
| Contact name |
wu hao |
| E-mail(s) |
haowu9711@gmail.com
|
| Phone |
19821361851
|
| Organization name |
Shanghai General Hospital
|
| Department |
Precision Research Center for Refractory Diseases, Institute for Clinical Research
|
| Street address |
No. 650 Xin Songjiang Road
|
| City |
shanghai |
| State/province |
shanghai |
| ZIP/Postal code |
201613 |
| Country |
China |
| |
|
| Platform ID |
GPL26167 |
| Series (1) |
| GSE209739 |
Effect of PDGF, TGF-β and miR-221 on gene expression during proliferation and differentiation of human aortic smooth muscle cells |
|
| Relations |
| BioSample |
SAMN29953739 |
| SRA |
SRX16660596 |
