|
| Status |
Public on Jul 17, 2024 |
| Title |
RNA-Seq_HEK293T_siKDM1A_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
HEK293T
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
cell type: Human embryonic kidney 293T cells
genotype: KDM1A knock down
treatment: siKDM1A
|
| Treatment protocol |
Normal condition
|
| Growth protocol |
HEK293T cells were cultured in DMEM medium supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy Mini Kit (Qiagen) following the manufacturer’s protocol. DNase I in column digestion was included to ensure the RNA quality.
RNA library preparation was performed by using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (E7420L). Paired-end sequencing was performed with Illumina HiSeq platform at RiboBio Co., Ltd. or Amogene Biotech Co., Ltd.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Basecalling was performed using CASAVA version 1.4
Reads were aligned to the hg19 genome assembly using STAR version 2.7.10.
Assembly: hg19
|
| |
|
| Submission date |
Jul 27, 2022 |
| Last update date |
Jul 17, 2024 |
| Contact name |
Jiancheng Ding |
| E-mail(s) |
djch@xmu.edu.cn
|
| Organization name |
Xiamen University
|
| Street address |
School of Pharmaceutical Sciences, Xiamen University Xiang`an South Road
|
| City |
Xiamen |
| State/province |
Fujian |
| ZIP/Postal code |
361102 |
| Country |
China |
| |
|
| Platform ID |
GPL21290 |
| Series (2) |
| GSE209909 |
CARM1 interacts and hypermethylates the NuRD chromatin remodeling complex to promote gene transcriptional activation and cell cycle progression |
| GSE209910 |
CARM1 interacts and hypermethylates the NuRD chromatin remodeling complex to promote gene transcriptional activation and cell cycle progression |
|
| Relations |
| BioSample |
SAMN30000075 |
| SRA |
SRX16695437 |
