|
| Status |
Public on Feb 09, 2011 |
| Title |
Blimp1 gfp/gfp Treg, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/B6
tissue: bone marrow
cell type: regulatory T cells
genotype: Blimp1 gfp/gfp
beadchip barcode: 1880222048
beadchip section: D
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from regulatory T cells were prepared with the RNeasy Micro kit (Qiagen). RNA quality was ascertained using the Agilent Bioanalyser 2100 with the NanoChip protocol.
|
| Label |
Cy3
|
| Label protocol |
We labeled up to 500 ng with Cy3 using the Total Prep RNA amplification kit (Ambion).
|
| |
|
| Hybridization protocol |
Labeled cRNA (1.5 μg) was prepared for hybridization to Illumina mouseWG-6 V1.1 (R3) BeadChips following the standard Illumina protocol. The bead arrays were washed and blocked in E1 buffer for 10 min in a rocking incubator and 10 additional minutes with 2 ml of E1 buffer. They were then stained with streptavidin-conjugated Cy3 fluorescent dye. The fluorescent reagent was washed away with E1BC solution. The arrays were finally air dried.
|
| Scan protocol |
Arrays were scanned by an Illumina BeadArray Reader (Illumina California USA). The default setting was used.
|
| Data processing |
Non-normalized summary probe profiles were output from BeadStudio and analyzed using the limma packages of the Bioconductor open-source software project (http://www.bioconductor.org). The raw intensities were normexp background corrected with offset 16, and normalized by the neqc function.
|
| |
|
| Submission date |
Feb 08, 2011 |
| Last update date |
Feb 09, 2011 |
| Contact name |
Wei Shi |
| E-mail(s) |
Wei.Shi2@monash.edu
|
| Organization name |
Monash University
|
| Street address |
Wellington Rd
|
| City |
Clayton |
| State/province |
Victoria |
| ZIP/Postal code |
3800 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE27143 |
Gene expression profiles of mouse Blimp1 +/gfp and Blimp1 gfp/gfp regulatory T cells |
|
