|
| Status |
Public on Jun 01, 2012 |
| Title |
Epithelial Killed WT N. meningitidis 3h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
16HBE14 human bronchial epithelial cells,3h,Killed WT N. meningitidis
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: 16HBE14 human bronchial epithelial cells
infection status: Killed WT N. meningitidis
time (hours): 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop 1000 instrument.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 1
|
| Data processing |
The data were analysed in Genespring GX7.3 software. Measurements less than 0.01 was set to 0.01. Data was then normalised at the 50th percentile.
|
| |
|
| Submission date |
Feb 28, 2011 |
| Last update date |
Jun 01, 2012 |
| Contact name |
Ahmad Nazri Mohamed Naim |
| E-mail(s) |
mohamedan@gis.a-star.edu.sg
|
| Phone |
65-98547654
|
| Organization name |
Genome institute of Singapore
|
| Department |
Infectious disease
|
| Lab |
lab 1
|
| Street address |
60 Biopolis Street, Genome, #02-01
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL5104 |
| Series (1) |
| GSE27557 |
Genome-wide gene expression analysis of 16HBE14 human bronchial epithelial cells in response to Neisseria |
|
