|
| Status |
Public on Apr 21, 2023 |
| Title |
genomic DNA from Fat from YoungIso3 |
| Sample type |
genomic |
| |
|
| Source name |
Fat from YoungIso3
|
| Organism |
Mus musculus |
| Characteristics |
age (years): 0.5
mouse strain: C57BL/6 WT
Sex: Female
mouse id: YoungIso3
tissue: Fat
|
| Treatment protocol |
Parabiosis was carried out as previously described (Zhang et al., 2021). Female C57Bl/6 mice were pre-screened to minimize body size differences, and were randomly assigned to parabiosis pairs. Isochronic pairs consisted of two 3-month-old or 20-month-old mice and heterochronic pairs consisted of one 3-month-old mouse and one 20-month-old mouse. Pairs were surgically attached and maintained for 3 months. Following 3 months of parabiosis, a subset of mice were euthanized for analysis and another subset were surgically separated. Fascia and skin were sutured closed following separation, and mice were allowed to recover for 2 months, after which they were for euthanized for analysis.
|
| Growth protocol |
This experiment was approved by the Duke University IACUC. C57Bl/6 mice were obtained from Jackson Laboratories and acclimated to our animal facility for at least 48 h before being subjected to any experimental manipulation. Aged C57Bl/6 mice for parabiosis experiments were obtained from the NIA aged rodent colony. Mice were maintained in a barrier facility in sterilized, ventilated cages and fed standard laboratory chow (LabDiet 5053) and reverse osmosis drinking water ad libitum and maintained on a 12h:12h light:dark cycle. Mice were generally housed socially. Mice were humanely euthanized at the conclusion of each experiment by CO2 exposure followed by cervical dislocation.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from whole blood using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol.
|
| Label |
Cy3,Cy5
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
Young isochronic parabiosis
|
| Data processing |
For mammalian microarray analysis, raw methylation data were first normalized and beta values were calculated using the SeSAMe R package.
|
| |
|
| Submission date |
Feb 02, 2023 |
| Last update date |
Jul 03, 2023 |
| Contact name |
Jesse Poganik |
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
| Department |
Medicine/Genetics
|
| Lab |
Gladyshev Lab
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL28271 |
| Series (2) |
| GSE224361 |
DNA methylation changes in mice induced by parabiosis and recovery [HorvathMammalMethylChip40] |
| GSE224447 |
DNA methylation and gene expression changes in mice induced by parabiosis and recovery |
|
