|
| Status |
Public on Apr 06, 2012 |
| Title |
dcp5-1 replicate dye-swap 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
dcp5-1
|
| Organism |
Arabidopsis |
| Characteristics |
age: 12-day old seedlings
treatment: time 0
|
| Treatment protocol |
Seedlings were transferred onto 3MM papers (Whatman) at 22°C exposed to 60% humidity at room temperature under dim light. After 30 min fresh weight of dehydrated seedlings was reduced to about 60%.
|
| Growth protocol |
Plants were grown on sterile solid half-strength Murashige-Skoog (MS) medium for 12 days (22°C, 16/8h photoperiod cycles).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using plant RNeasy mini kit (Qiagen) following manufacturer's instructions
|
| Label |
cy5
|
| Label protocol |
100ng of each sample was labeled together with two-color spike in RNAs using Lowinput quickamp labeling kit (Agilent)
|
| |
|
| Channel 2 |
| Source name |
dcp5-1
|
| Organism |
Arabidopsis |
| Characteristics |
age: 12-day old seedlings
treatment: dehydration 30min
|
| Treatment protocol |
Seedlings were transferred onto 3MM papers (Whatman) at 22°C exposed to 60% humidity at room temperature under dim light. After 30 min fresh weight of dehydrated seedlings was reduced to about 60%.
|
| Growth protocol |
Plants were grown on sterile solid half-strength Murashige-Skoog (MS) medium for 12 days (22°C, 16/8h photoperiod cycles).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using plant RNeasy mini kit (Qiagen) following manufacturer's instructions
|
| Label |
cy3
|
| Label protocol |
100ng of each sample was labeled together with two-color spike in RNAs using Lowinput quickamp labeling kit (Agilent)
|
| |
|
| |
| Hybridization protocol |
hybridization buffer (Agilent Gene expression hybridization kit) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially in Buffer 1 and Buffer 2.
|
| Scan protocol |
Scanned on an Agilent G2565A scanner. Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Apr 06, 2012 |
| Contact name |
Jun Xu |
| E-mail(s) |
jxu@mail.rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Street address |
1230 york avenue
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE28493 |
dehydration response: dcp5-1 and WT |
|
