|
| Status |
Public on Nov 28, 2023 |
| Title |
Mutant ZmMYBR29, replicate 3 |
| Sample type |
SRA |
| |
|
| Source name |
Endosperm
|
| Organism |
Zea mays |
| Characteristics |
tissue: Endosperm
gene id: Zm00001d006791
cultivar: B73
age: 8 DAP
genotype: mutant
|
| Growth protocol |
Wild type and mutant plant kernels cultivated in the greenhouse of China Agricultural University (Beijing, China)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
5-10 of 8 DAP endosperms of wild type and mutant were pooled for extracting total RNA with an RNAprep Pure Plant Kit (cat. no. DP441, TIANGEN)
MP3RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
8DAPMU
|
| Data processing |
Trimmomatic (version 0.39) was used to trim the sequence reads and Hisat2 (version 2.2.1) was used to align them to the B73 v4 reference genome.
Supplementary files format and content: Zea mays ssp mays B73 v4
Supplementary files format and content: Matrix table with raw counts for every sample (column) and gene (rows)
|
| |
|
| Submission date |
Apr 18, 2023 |
| Last update date |
Nov 28, 2023 |
| Contact name |
Qiang Huo |
| E-mail(s) |
huoqiang@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Street address |
Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100091 |
| Country |
China |
| |
|
| Platform ID |
GPL25410 |
| Series (2) |
| GSE201701 |
Decoding the gene regulatory network of endosperm differentiation in maize |
| GSE229942 |
Decoding the gene regulatory network of endosperm differentiation in maize [MP3RNA-seq] |
|
| Relations |
| BioSample |
SAMN34232758 |
| SRA |
SRX20378050 |
