|
| Status |
Public on Jun 19, 2023 |
| Title |
N_PE2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Surrounding non-malignant tissue
|
| Organism |
Homo sapiens |
| Characteristics |
hpv: no
tissue: Surrounding non-malignant tissue
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from frozen penile tissue was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA)
|
| Label |
Cy3
|
| Label protocol |
This step of the methodology follow manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
pool of five normal glans obtained from necropsies
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from frozen penile tissue was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA)
|
| Label |
Cy5
|
| Label protocol |
This step of the methodology follow manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
This step of the methodology follow manufacturer's instructions
|
| Scan protocol |
The array images were acquired using the Scan Control (version 8.1) software program (Agilent Technologies, CA, USA). Images were quantified using Agilent Feature Extraction Software version v.10.1.1.1 (Agilent Technologies, CA, USA)
|
| Description |
Surrounding non-malignant tissue
Surrounding non-malignant tissue
|
| Data processing |
Agilent Feature Extraction Software (v. 10.1.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 16, 2023 |
| Last update date |
Jun 19, 2023 |
| Contact name |
Fabio Albuquerque Marchi |
| E-mail(s) |
fabio.marchi@fm.usp.br
|
| Organization name |
ICESP
|
| Department |
CTO
|
| Street address |
Av Dr Arnaldo
|
| City |
Sao Paulo |
| ZIP/Postal code |
01246-000 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL19930 |
| Series (1) |
| GSE235188 |
Genome-wide DNA methylation study in penile cancer |
|
