|
| Status |
Public on Sep 23, 2013 |
| Title |
Wild_type_Arabidopsis_3wk_seedling-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type_3wk_seedllings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: wt
age: 3 weeks
tissue: whole seedlings
|
| Growth protocol |
All plants were grown in MS (1% sucrose) agar media in 16hr light / 8hr dark cycle in 23 Celsius degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy3
|
| Label protocol |
1 µg of total RNA was used for labeling procedure using QickAmp Labeling kit (One-color) (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized using Gene Expression Hybridization kit (Agilent) and washed by Gene Expression wash pack (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
|
| Description |
wt_rep3
Many seedlings were grown in one plate and several of them were harvested for RNA extraction
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction. After that, signal of each probe was divided by median value of filter-passed probes.
|
| |
|
| Submission date |
Sep 06, 2011 |
| Last update date |
Sep 23, 2013 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
nobutaka.mitsuda@aist.go.jp
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL12621 |
| Series (2) |
| GSE31886 |
Transcriptomes of 35S:MYB106VP16 and wild-type Arabidopsis seedling |
| GSE31887 |
Functional analysis of Arabidopsis MYB106 and WIN1/SHN1 transcription factors |
|
