|
| Status |
Public on Sep 20, 2011 |
| Title |
BT474-J4_Lap-GSK089_24-2 |
| Sample type |
RNA |
| |
|
| Source name |
BT474-J4 breast cancer cell line, lapatinib and foretinib
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BT474-J4
cell type: breast cancer
lapatinib sensitivity: acquired lapatinib-resistant
her2 status: HER2-positive
treatment: lapatinib and foretinib
|
| Treatment protocol |
Cells were treated with vehicle (DMSO), 1 µM lapatinib, 0.1 µM GSK1363089 (foretinib) or a combination of 1µM lapatinib and 0.1 µM foretinib for 24h.
|
| Growth protocol |
Cells were plated in RPMI medium containing 10% FBS overnight at 37ºC, 5%CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Response Genetics, Inc.)
|
| |
|
| Hybridization protocol |
Experiment conducted at Response Genetics, Inc.
|
| Scan protocol |
Experiment conducted at Response Genetics, Inc.
|
| Description |
Gene expression data of a breast cancer cell line.
|
| Data processing |
RNA microarray signals were normalized by MAS5. QC and data analysis were performed using Omicsoft microarray analysis tools (http://www.omicsoft.com/).
|
| |
|
| Submission date |
Sep 20, 2011 |
| Last update date |
Sep 20, 2011 |
| Contact name |
Yuan Liu |
| Fax |
610-917-5000
|
| Organization name |
GlaxoSmithKline
|
| Department |
Biomarker
|
| Street address |
1250 South Collegeville Rd
|
| City |
Collegeville |
| State/province |
PA |
| ZIP/Postal code |
19446 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16179 |
BT474 and BT474-J4 microarray data |
|
