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Status |
Public on Jun 01, 2012 |
Title |
WT ABA+DEX induced 2 |
Sample type |
RNA |
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Source name |
whole seedling
|
Organism |
Arabidopsis thaliana |
Characteristics |
developmental stage according to boyes et. al 2001: 1.02 genotype/variation: WT, Col-0 treatment: 4h treatment with ABA and DEX
|
Treatment protocol |
Two weeks old seedlings of wildtype (WT, Col-0) and ABI3:GR transgenics were incubated in liquid MS media supplemented with either Abscisic acid (ABA;10 µM) or Dexamethasone (DEX; 30 µM) or both; an identical volume of ethanol used as solute for ABA and DEX was added in the mock treatment
|
Growth protocol |
Seedlings were grown on MS-agar plates, supplemented with selective antibiotic, 16 h light/ 8h dark
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
Cyanin 3 labeled cRNA was prepared from 100 ng total RNA using the Low Input Quick Amp Labeling Kit, One color (#5190-2305) of Agilent Technologies according to the manufacturers instructions
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|
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Hybridization protocol |
1.65 µg of Cy3 labelled cRNA was fragmented according to the manufacturer's instruction. 1.425 µg of fragmented cRNA (conc:15 ng/µl) was hybridized at 65°C, 10 rpm, 17 h in an Agilent hybridization oven
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Scan protocol |
Scanning was performed using an Agilent DNA Microarray Scanner (Model G2505C) and Scan Control Software (version A.8.3.1) of Agilent
|
Description |
gene expression after 4h treatment with ABA and DEX
|
Data processing |
Primary data analysis was performed using Agilent's Feature Extraction Software (version 10.7.3.1)
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Submission date |
Nov 25, 2011 |
Last update date |
Jun 01, 2012 |
Contact name |
Gudrun Moenke |
E-mail(s) |
moenke@ipk-gatersleben.de
|
Organization name |
IPK
|
Street address |
Corrensstr. 3
|
City |
Gatersleben |
ZIP/Postal code |
06466 |
Country |
Germany |
|
|
Platform ID |
GPL12621 |
Series (2) |
GSE33951 |
Identification of target genes of ABI3 [Agilent 44k] |
GSE34043 |
Identification of target genes of ABI3 |
|