|
| Status |
Public on Dec 10, 2011 |
| Title |
Healthy 0 h Control (Cy5)_O3 48 h (Cy3)_Pooled 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Ozone fumigated 48 h
|
| Organism |
Oryza sativa |
| Characteristics |
cultivar: Nipponbare
age: 2 wk
treatment: ozone
time: 48h
|
| Treatment protocol |
03, 0.2 ppm
|
| Growth protocol |
Plants were grown in growth chamber in soil for 14 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit: QIAGEN, Rneasy Mini
|
| Label |
Cy3
|
| Label protocol |
Manual: Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| Channel 2 |
| Source name |
Ozone Control 0 h
|
| Organism |
Oryza sativa |
| Characteristics |
cultivar: Nipponbare
age: 2 wk
treatment: control
time: 0h
|
| Treatment protocol |
control, filtered
|
| Growth protocol |
Plants were grown in growth chamber in soil for 14 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit: QIAGEN, Rneasy Mini
|
| Label |
Cy5
|
| Label protocol |
Manual: Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| |
| Hybridization protocol |
hybridization_Kit: in situ hybridization plus
|
| Scan protocol |
GenePix 4000B
|
| Description |
GPL892
|
| Data processing |
Genepix ver. 4.0 quantitative microarray analysis application program
|
| |
|
| Submission date |
Dec 08, 2011 |
| Last update date |
Dec 10, 2011 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
|
| Phone |
+81-(0)90-1853-7875
|
| Organization name |
University of Tsukuba
|
| Department |
Institute of Health and Sport Sciences
|
| Lab |
Global Sport Innovation (GSI) 403
|
| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE34256 |
Global Identification of Potential Gene Biomarkers Associated with Ozone-induced Foliar Injury in Rice Seedling Leaves by Correlating their Symptom Severity with Transcriptome Profiling |
|
