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Sample GSM845921 Query DataSets for GSM845921
Status Public on Dec 10, 2011
Title Healthy 0 h Control (Cy3)_O3 72 h (Cy5)_Pooled 1
Sample type RNA
 
Channel 1
Source name Ozone Control 0 h
Organism Oryza sativa
Characteristics cultivar: Nipponbare
age: 2 wk
treatment: control
time: 0h
Treatment protocol control, filtered
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol kit: QIAGEN, Rneasy Mini
Label Cy3
Label protocol Manual: Low RNA Input Linear Amplification kit standard protocol
 
Channel 2
Source name Ozone fumigated 72 h
Organism Oryza sativa
Characteristics cultivar: Nipponbare
age: 2 wk
treatment: ozone
time: 72h
Treatment protocol 03, 0.2 ppm
Growth protocol Plants were grown in growth chamber in soil for 14 days
Extracted molecule total RNA
Extraction protocol kit: QIAGEN, Rneasy Mini
Label Cy5
Label protocol Manual: Low RNA Input Linear Amplification kit standard protocol
 
 
Hybridization protocol hybridization_Kit: in situ hybridization plus
Scan protocol GenePix 4000B
Description GPL892
Data processing Genepix ver. 4.0 quantitative microarray analysis application program
 
Submission date Dec 08, 2011
Last update date Dec 10, 2011
Contact name RANDEEP RAKWAL
E-mail(s) plantproteomics@gmail.com
Phone +81-(0)90-1853-7875
Organization name University of Tsukuba
Department Institute of Health and Sport Sciences
Lab GSI 403
Street address 1-1-1 Tennodai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8574
Country Japan
 
Platform ID GPL892
Series (1)
GSE34256 Global Identification of Potential Gene Biomarkers Associated with Ozone-induced Foliar Injury in Rice Seedling Leaves by Correlating their Symptom Severity with Transcriptome Profiling

Data table header descriptions
ID_REF
VALUE -INV_VALUE: Log 10 based (normalized ch2 intensity / normalized ch1 intensity)
Norm_Ch1 Normalized (lowess and rank consistency) signal of ch1
Norm_Ch2 Normalized (lowess and rank consistency) signal of ch2
Raw_Ch1 The net CH1 signal following the subtraction of the background from the raw mean CH1 signal
Raw_Ch2 The net CH2 signal following the subtraction of the background from the raw mean CH2 signal
INV_VALUE Log 10 based (normalized ch1intensity / normalized ch2 intensity)

Data table
ID_REF VALUE Norm_Ch1 Norm_Ch2 Raw_Ch1 Raw_Ch2 INV_VALUE
1 1.39 444 18 425 15 -1.39
2 1.33 405 19 391 16 -1.33
3 0.54 7 2 7 0 -0.54
4 0.37 42 18 41 16 -0.37
5 0.55 53 15 53 13 -0.55
6 0.34 13 6 13 4 -0.34
7 0.54 28 8 27 8 -0.54
8 -0.09 46 57 46 60 0.09
9 0.48 12 4 13 3 -0.48
10 0.7 20 4 19 4 -0.70
11 0.96 92 10 92 6 -0.96
12 0.04 111 101 109 101 -0.04
13 0.18 33 22 32 22 -0.18
14 0.83 135 20 138 18 -0.83
15 0.08 71 59 72 56 -0.08
16 0.38 12 5 10 3 -0.38
17 0.32 58 28 59 26 -0.32
18 0.53 64 19 67 17 -0.53
19 0.19 14 9 14 7 -0.19
20 0.39 47 19 47 16 -0.39

Total number of rows: 22575

Table truncated, full table size 613 Kbytes.




Supplementary file Size Download File type/resource
GSM845921_O3__132_.gpr.gz 1.3 Mb (ftp)(http) GPR
Processed data included within Sample table
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