|
| Status |
Public on Oct 18, 2012 |
| Title |
Recombinant 14-028 |
| Sample type |
RNA |
| |
|
| Source name |
infected culture
|
| Organisms |
Chlorocebus aethiops; Alphavirus |
| Characteristics |
cell line: Vero
infection: SIN/ZPC
purpose: Validation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using Trizol extraction protocol
|
| Label |
Cy3
|
| Label protocol |
amino-allyl Cy3 dye coupled to cDNA
|
| |
|
| Hybridization protocol |
70 degree incubation for 12 hours
|
| Scan protocol |
scanned using GenePix 4000B scanner
|
| Description |
hybridization of infected culture for use in validating VIPR HMM
|
| Data processing |
None. Regarding the submission of raw (not normalized) data, the microarray data provided represents an unconventional use of microarrays as a diagnostic tool as opposed to the conventional use of microarrays to study gene expression patterns. Each software tool that has been developed to analyze diagnostic microarray data employs its own normalization method. Thus, the standard input to these programs is non-normalized data which is then independently processed by whatever software tool is chosen.
|
| |
|
| Submission date |
Dec 16, 2011 |
| Last update date |
Oct 18, 2012 |
| Contact name |
Adam F Allred |
| E-mail(s) |
adam.f.allred@gmail.com
|
| Organization name |
Washington University in St. Louis, School of Medicine
|
| Street address |
660 S. Euclid
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63139 |
| Country |
USA |
| |
|
| Platform ID |
GPL15022 |
| Series (1) |
| GSE34490 |
VIPR HMM: A hidden Markov model for detecting recombination with microbial detection arrays |
|
