|
| Status |
Public on Jan 12, 2012 |
| Title |
individual 32, D treatment |
| Sample type |
RNA |
| |
|
| Source name |
Invasive cancer_DPN
|
| Organism |
Homo sapiens |
| Characteristics |
tissue origin: Invasive cancer
breast cancer type: ER+/Her2-
cell type: cells forming mammospheres
treatment: estrogen receptor beta activation by DPN
|
| Treatment protocol |
Mammospheres are treated with D (DPN), T (4OHT) or untreated (vehicle control).
|
| Growth protocol |
Breast cancer stem cells were isolated and propagated from newly resected breast cancers. Cells forming mammospheres were used for experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was amplified using NuGEN Ovation Pico WTA System followed by generation of ST-cDNA using WT Ovation Exon Module.
|
| Label |
biotin
|
| Label protocol |
Fragmentation and labeling were performed using NuGen Encore Biotin Module.
|
| |
|
| Hybridization protocol |
Fragmented and labeled ST-cDNA was hybridized to HuGen 1.0 ST arrays and washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
32D
gene expression data from ligand treated mammospheres
|
| Data processing |
Preprocessing was performed in Affymetrix Expression Console (v. 1.1) using the following methods: Summarization: Median Polish (RMA), Normalization: Sketch-Quantile.
|
| |
|
| Submission date |
Jan 11, 2012 |
| Last update date |
Jan 12, 2012 |
| Contact name |
Johan Hartman |
| E-mail(s) |
johan.hartman@ki.se
|
| Organization name |
Karolinska Institute
|
| Department |
Dept of Oncology and Pathology
|
| Street address |
R8:03, CCK
|
| City |
Stockholm |
| ZIP/Postal code |
SE-171 77 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35031 |
Estrogen receptors in breast cancer stem cells |
|
