|
| Status |
Public on May 09, 2012 |
| Title |
Desmoplastic fibroblastoma 3 |
| Sample type |
RNA |
| |
|
| Source name |
soft tissue
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: tumor biopsy
tissue: desmoplastic fibroblastoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the TRIzol reagent (Invitrogen) and purified with Qiagen´s RNeasy kit according to the manufacturers´ instructions.
|
| Label |
biotin
|
| Label protocol |
cDNA was labelled with TdT using the WT Terminal Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Biotinylated cDNA was hybridized to GeneChip Gene 1_0 st arrays. Arrays were washed and stained on an Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanning was carried out with the GeneChip Scanner 3000 (Affymetrix).
|
| Description |
DF_case_3
Global gene expression data
|
| Data processing |
Image analysis was perfomed using the GeneChip Operating Software (GCOS, Affymetrix). Background correction, normalization and probe summarization was done with the robust multichip average (RMA) algorithm implemented in the Expression Console software (Affymetrix).
|
| |
|
| Submission date |
Jan 17, 2012 |
| Last update date |
May 09, 2012 |
| Contact name |
Karolin H Nord |
| Organization name |
Lund University
|
| Department |
Dept of Clinical Genetics
|
| Street address |
BMC C13
|
| City |
Lund |
| ZIP/Postal code |
SE-22184 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35162 |
FOSL1 as a candidate target gene for 11q12 rearrangements in desmoplastic fibroblastoma |
|
