|
| Status |
Public on Jan 03, 2013 |
| Title |
667N microRNA |
| Sample type |
RNA |
| |
|
| Source name |
normal colon mucosa, patient 667
|
| Organism |
Homo sapiens |
| Characteristics |
age (yr): 78
gender: Female
location: Transverse colon
t: 4
n: 0
m: 0
Stage: II
differentiation: moderate
tissue: normal colorectal tissue
individual: patient 667
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and isolated using mirVana TM RNA Isolation Kit (Applied Biosystem p/n AM1556) following the manufacture’s introduction. The concentration of RNA was measured by NanoDrop spectrophotometer. Total RNA concentration ranged form 100ng/ul to 1ug/ul. The RNA quality was evaluated by Agilent 2100 Bioanalyzer; RNAs with RIN>=7.0 and 28S/18S>0.7 were accepted for microarray analysis.
|
| Label |
cy3
|
| Label protocol |
100ng of total RNA was reverse transcribed into cDNA and labeled with Cy3, and then hybridized on a miRNA array (Agilent-021827 Human miRNA Microarray (V3)) using Agilent’s miRNA Complete Labeling and Hyb Kit (p/n 5190-0456).
|
| |
|
| Hybridization protocol |
100ng of total RNA was reverse transcribed into cDNA and labeled with Cy3, and then hybridized on a miRNA array (Agilent-021827 Human miRNA Microarray (V3)) using Agilent’s miRNA Complete Labeling and Hyb Kit (p/n 5190-0456).
|
| Scan protocol |
The slides were then scanned in microarray scanner by Agilent scan control software Version A7.0
|
| Description |
miRNA expression of normal colon mucosa
|
| Data processing |
The data collection, background subtraction and with-in array normalization were carried out by Agilent Feature Extraction (FE) software version 9.5.3.
|
| |
|
| Submission date |
Feb 21, 2012 |
| Last update date |
Jan 03, 2013 |
| Contact name |
Jihong Fu |
| E-mail(s) |
fjh720@126.com
|
| Organization name |
Xinhua Hospital
|
| Department |
colorectal surgery department
|
| Street address |
Kongjiang Road 1665, Shanghai, 200092, China
|
| City |
shanghai |
| State/province |
shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
|
| Platform ID |
GPL14767 |
| Series (1) |
| GSE35982 |
Identifying microRNA-mRNA regulatory networks in colorectal cancer by a combination of expression profile and bioinformatics analysis |
|
