|
| Status |
Public on Jan 03, 2013 |
| Title |
Identifying microRNA-mRNA regulatory networks in colorectal cancer by a combination of expression profile and bioinformatics analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
A multi-step approach combining microarray profile and bioinformatics analysis was adopted to identify the CRC specific miRNA-mRNA regulatory network. First, differentially expressed miRNAs and mRNAs were found out in CRC samples compared with normal epithelial tissues by miRNA and mRNA microarray respectively. Secondly the target mRNAs of dysregualted miRNA were identified by a combination of Pearson correlation coefficient between the expression level of miRNAs and mRNAs and online miRNA target predicting databases. Thirdly, the biological pathways which the miRNA-mRNA pairs involved in were identified by DAVID. Finally, some of the dysregualted miRNAs and mRNAs were validated by qRT-PCR
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| |
|
| Overall design |
RNA isolated from 8 colorectal cancer tissues and their corresponding adjacent normal tissues were analyzed.
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| |
|
| Contributor(s) |
Fu J, Tang W, Du P, Wang G, Chen W, Li J, Zhu Y, Gao J, Cui L |
| Citation(s) |
22703586 |
| |
| Submission date |
Feb 21, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Jihong Fu |
| E-mail(s) |
fjh720@126.com
|
| Organization name |
Xinhua Hospital
|
| Department |
colorectal surgery department
|
| Street address |
Kongjiang Road 1665, Shanghai, 200092, China
|
| City |
shanghai |
| State/province |
shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
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| Platforms (2) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
| GPL14767 |
Agilent-021827 Human miRNA Microarray G4470C (Feature Number version) |
|
| Samples (32)
|
|
| Relations |
| BioProject |
PRJNA151639 |
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