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Status |
Public on Feb 01, 2020 |
Title |
Wild-type 7-day-old seedlings Replicate 1 |
Sample type |
RNA |
|
|
Source name |
Col0 seedlings
|
Organism |
Arabidopsis thaliana |
Characteristics |
ecotype: Columbia ecotype organ: seedlings age: 7-day-old genotype/variation: wild type
|
Growth protocol |
Plants were grown in the greenhouse under 16h light and 8h dark at 22°C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the Trizol reagent (Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up according to manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
2 µg of total RNA were labeled according Aligent's instructions. Please see manufacture’s web site at http://www.aligent.com/
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|
Hybridization protocol |
Please see manufacture’s web site at http://www.aligent.com/
|
Scan protocol |
Scanned on an Agilent G2565BA scanner. Images were quantified using Agilent Feature Extraction Software.
|
Description |
Biological replicate 1 of Col0 seedlings
|
Data processing |
Agilent Feature Extraction Software was used for background subtraction. Signal intensity were normalized using Quantile method. Signal : raw Cy3 signal intensity ; Log2NormSignal : Log2 transformed Normalized Signal
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Submission date |
Aug 06, 2012 |
Last update date |
Feb 01, 2020 |
Contact name |
Wen-Hui Shen |
E-mail(s) |
wen-hui.shen@ibmp-cnrs.unistra.fr
|
Phone |
+33 3 67 15 53 26
|
Fax |
+33 3 88 61 44 42
|
Organization name |
IBMP-CNRS
|
Street address |
12 rue du General Zimmer
|
City |
Strasbourg |
ZIP/Postal code |
67084 |
Country |
France |
|
|
Platform ID |
GPL12621 |
Series (1) |
GSE94731 |
Arabidopsis thaliana Wild-type vs. mutant (PcG) |
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