|
| Status |
Public on Mar 01, 2013 |
| Title |
LFYasEMF1_15D_SD |
| Sample type |
RNA |
| |
|
| Source name |
15D old LFYasEMF1 seedlings grown under short day
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: LFYasEMF1
treatment: grown under short day
tissue: whole seedlings
age: 15 days
|
| Treatment protocol |
Seedings of WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 were harvested at 7 days and 15 days.
|
| Growth protocol |
Seeds of WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 were saw on 0.8% 1/2 MS agar plate containing 1.5% sucrose and seedlings were grown under short day condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Alexa Flour 555
|
| Label protocol |
One microgram total RNA of each sample was used as a template to generate amino allyl modified RNA (aRNA), respectively. The amplified aRNA was labeled with Alexa Flour 555 reactive dyes (Invitrogen) and fragmented according to the manual instruction of the Amino Allyl MessageAmp aRNA Amplification Kit (Ambion) before hybridization to an Arabidopsis Gene Expression Microarray chip version 4.
|
| |
|
| Hybridization protocol |
According to the instruction of Agilent One-Color Microarray-Based Gene Expression Analysis Protocol, version 6.5, the chips were hybridized with equal amounts (1.65 μg) of Alexa 555 labeled aRNA derived from different samples for 17 h at 65 °C and washed according to the manufacturer’s instructions of the Agilent Gene Expression Hybridization Kit and Gene Expression Wash Pack.
|
| Scan protocol |
Chip images were scanned using the Agilent DNA microarray scanner (G2565CA, Agilent Technologies).
|
| Description |
Gene expression of 15D old seedlings grown under short day condition
|
| Data processing |
The images were analyzed by the use of the Feature Extraction software (version 10.7.1.1) following the GE1-107_Sep09 protocol (Agilent Technologies). Signal intensities were Quantile normalized using GeneSpring 11.2 software.
|
| |
|
| Submission date |
Aug 07, 2012 |
| Last update date |
Mar 01, 2013 |
| Contact name |
Mao-Sen Liu |
| E-mail(s) |
d89226001@ntu.edu.tw
|
| Organization name |
Institute of Plant and Microbial Biology, Academia Sinica, Taiwan
|
| Street address |
128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE39947 |
Arabidopsis ult1-3 mutant restored LFYasEMF1's phenotype |
|
