GEO DataSets

(Submitter supplied) In animals, the maternal-to-embryonic transition (MET) is an important step occurring in the first days of early development. This important transition involves the degradation of maternal transcripts that have been stocked during oogenesis and used until this transition. Moreover, some precise and specific control mechanisms must govern the adequate synchronization of the MET events to promote embryonic genome activation. more...

Organism:

Symphalangus syndactylus; Gorilla gorilla; Pongo abelii; Homo sapiens; Sus scrofa; Cricetulus griseus; Mus musculus; Saguinus imperator; Pan troglodytes; Bos taurus; Ovis aries; Rattus norvegicus; Lemur catta; Lagothrix; Macaca nemestrina; Canis lupus familiaris; Rhinopithecus bieti; Ateles sp.; Macaca mulatta; Pan paniscus; Monodelphis domestica

Type:

Expression profiling by array

Platform:

GPL15194

1 Sample

Download data: TXT

(Submitter supplied) In summary the main goal of this study is to determine the miRNA transcriptional profile of bovine endoemtrium at early stage of development in relation to pregnancy success after transfer of in vivo and in vitro derived blastocysts

Organism:

synthetic construct; Bos taurus

Type:

Non-coding RNA profiling by array

Platform:

GPL16384

24 Samples

Download data: CEL

(Submitter supplied) Microarray analysis was performed in ooplast samples to identify gene expression profiles associated with the embryo capacity to develop to blastocyst stage

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13515

32 Samples

Download data: GPR

(Submitter supplied) Pre-implantation embryos derived by in vitro fertilization differ in their developmental potential from embryos obtained in vivo. In order to characterize changes in gene expression profiles caused by in vitro culture environment, we employed microarray constructed from bovine embryo-specific cDNAs (BlueChip v., Université Laval, Québec) to compare in vitro cultured and in vivo derived bovine embryos at 4-cell and 8-cell stage.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11061

6 Samples

Download data: MEV

(Submitter supplied) Blastocyst formation is a primordial event of pre-implantation development since it is required for pregnancy establishment and progression. The blastocyst plays a pivotal role because it is the stage at which the embryo is transferred and starts coordinated cross-talks with the mother. It is also the terminal step of pre-implantation developmentbefore transfer; it reflects all stresses the embryo may have faced during the process of in-vitro treatment. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11247

9 Samples

Download data: TXT

(Submitter supplied) In vitro production of cattle embryos over the past two decades has revealed several negative impacts, which have been attributed to the artificial microenvironment. Studies of embryos produced in vitro clearly point to aberrant gene expression levels. So far, the causal association between phenotype and measured gene expression has not led to substantial improvement of in vitro production systems. The aim of the present study was to generate a unique dataset composed of microarray-derived relative transcript abundance values for blastocysts produced in ten in vitro systems differing primarily in culture medium formulation. Between-group comparisons allowed us to determine the level of overall similarity between systems relative to in vivo reference embryos. The use of the dataset to contrast all in vitro treatments with the in vivo blastocysts pointed to a single common gene network. The “boutique” array contained a panel of novel uncharacterized transcripts that were expressed variably depending on the medium in which the blastocysts were produced. These novel transcripts were expressed differentially in blastocysts even as carry-over from conditions encountered seven days earlier during oocyte maturation. All of the selected novel candidates that were thus expressed were from intergenic regions. The function of this long non-coding RNA remains unknown but points clearly to an additional level of complexity in early embryo development.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13261

65 Samples

Download data: TXT

(Submitter supplied) RNA separation in function of the poly(A) tail length: A- (A≤50) and A150 (A≥150) from 200 immature oocytes (at the germinal vesicle stage, GV) in quadruplicates

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

4 Samples

Download data: TXT

(Submitter supplied) There is an inevitable need for combining in vitro and in vivo culture systems during specific developmental time points to facilitate a comprehensive understanding of early embryo development which would yield insights into the molecular pathways controlling early development and to improve our knowledge in regulation of embryonic development. In current study, we aimed to understand the influences of alternative culture conditions (in vivo or in vitro) during EGA event on embryonic developmental rate, gene expression pattern and subsequent influences on pathways and biological functions controlling bovine embryo development.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

30 Samples

Download data: TXT

(Submitter supplied) Recently, microRNAs (miRNAs) have emerged as new players in the fine tuning of embryo development and implantation in mammals via posttranscriptional gene regulation mechanisms. Applying custom made multispecies arrays we aimed to analyze expression profile of microRNAs in peri-implantation porcine conceptuses/trophoblasts to identify their potential role at the maternal-fetal interface during the critical period of maternal recognition of pregnancy and implantation.

Organism:

Sus scrofa; Mus musculus; Homo sapiens; Bos taurus

Type:

Non-coding RNA profiling by array

Platform:

GPL18981

29 Samples

Download data: TXT

(Submitter supplied) In cattle, almost all fully grown vesicle stage oocytes (GV) have the ability to resume meisos, develop to Metaphase II stage (MII), support fertilization and progress through the early embryonic cycles in vitro. Yet without intensive selection, the majority fail to develop to the blastocyst stage. Using the Affymetrix Bovine Genome Array, global mRNA expression analysis of immature (GV) and in vitro matured (IVM) bovine oocytes was carried out to characterize the transcriptome of the bovine oocyte and to identify the key pathways associated with oocyte meiotic maturation and developmental potential.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL15840 GPL15841

30 Samples

Download data: TXT

(Submitter supplied) The study aimed to characterize miRNA expression in rainbow trout ovary during ovarian development from immature to mature stages. Whole ovary were collected at the following stages: immature pre-vitelogenesis (IMM), mid-vitellogenesis (MV), late vitellogenesis (LV), post-vitellogenesis (PV) and mature while meiotic maturation is in progress (MAT).

Organism:

Oncorhynchus mykiss

Type:

Non-coding RNA profiling by array

Platform:

GPL15841

14 Samples

Download data: TXT

(Submitter supplied) The study aimed to characterize gene expression in rainbow trout ovary during ovarian development from immature to mature stages. Whole ovary were collected at the following stages: immature pre-vitelogenesis (IMM), mid-vitellogenesis (MV), late vitellogenesis (LV), post-vitellogenesis (PV) and mature while meiotic maturation is in progress (MAT).

Organism:

Oncorhynchus mykiss

Type:

Expression profiling by array

Platform:

GPL15840

16 Samples

Download data: TXT

(Submitter supplied) During maternal-to-embryonic transition control of embryonic development gradually switches from maternal RNAs and proteins stored in the oocyte to gene products generated after embryonic genome activation (EGA). Detailed insight into the onset of embryonic transcription is obscured by the presence of maternal transcripts. Using the bovine model system, we established by RNA sequencing a comprehensive catalogue of transcripts in germinal vesicle and metaphase II oocytes, and in embryos at the 4-cell, 8-cell, 16-cell and blastocyst stages. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15750

18 Samples

Download data: TXT

(Submitter supplied) The objective of the present study was to use our own fabricated bovine embryo cDNA microarray to profile genome-wide expression patterns of genes during the implantation period. Duplicate readings were obtained by hybridization of a Cy3/Cy5 probe mixture for each sample. Data normalization was performed the local background intensity of each array spot was smoothed by the local weight regression (lowess) smoother and subtracted from spot intensity data. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS1003

Platform:

GPL1221

11 Samples

Download data: TIFF

Expression profiling of embryos at various days of gestation up to 21 days and fetuses and fetal membranes at 27 and 28 days of gestation. Results provide insight into the regulation of embryonic, fetal, and fetal membrane differentiation.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 7 development stage, 3 tissue sets

Platform:

GPL1221

Series:

GSE1414

11 Samples

Download data: TIFF

(Submitter supplied) Small RNAs play important roles in early embryonic development. However, their expression dynamics and modifications are poorly understood because of the scarcity of RNA that is obtainable for sequencing analysis. Using an improved deep sequencing method that requires as little as 10 ng of total RNA or 50 oocytes, we profile small RNAs in mouse oocytes and early embryos. We find that microRNA (miRNA) expression starts soon after fertilization, and the mature miRNAs carried into the zygote by sperm during fertilization are relatively rare compared to the oocyte miRNAs. more...

Organism:

Homo sapiens; Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL13112 GPL11154

26 Samples

Download data: TXT

(Submitter supplied) This study provides the first comprehensive analysis of gene expression and transcriptome dynamics of bovine metaphase II oocytes and in vivo developing bovine embryos. For this study, Affymetrix GeneChip Bovine Genome Array which covers ~23,000 transcripts was used, which revealed several distinct clusters of genes regulated during various stages of bovine preimplantation development. Keywords: Time course

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

23 Samples

Download data: CEL

(Submitter supplied) During mammalian pre-implantation embryonic development dramatic and orchestrated changes occur in gene transcription. The identification of the complete changes has not been possible until the development of the Next Generation Sequencing Technology. Here we report the first transcriptome dynamics of single matured bovine oocytes and all stages of pre-implantation embryos developed in vivo. Surprisingly, nearly half of the bovine genome, 11,488 to 12,729 genes involved in more than 100 pathways, is expressed in oocytes and early embryos. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18907

16 Samples

Download data: TXT

(Submitter supplied) The objective of the study was to compare the transcriptome of the bovine oocyte according to the chromatin configuration of the germinal vesicule

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

16 Samples

Download data: TXT