GEO DataSets

Analysis of embryonic fibroblast-like (EF) differentiated cells derived from H9 embryonic stem cells. Foreskin fibroblasts, and undifferentiated H9 and embryonic carcinoma cells also examined. Results provide insight into the differences between EF cells and fibroblasts at the molecular level.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 cell type sets

Platform:

GPL2700

Series:

GSE5416

4 Samples

Download data: TXT

(Submitter supplied) Mouse embryonic fibroblasts stimulated with varying doses of FGF2 in conventional hES cell medium were analysed on whole-genome expression chips to reveal altered expression of genes encoding secreted proteins. Keywords: Growth factor stimulation experiment

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2421

Platform:

GPL4055

4 Samples

Download data: TXT

(Submitter supplied) Mouse embryonic fibroblasts stimulated with 40 ng/ml of FGF2 in conventional hES cell medium were analysed on whole-genome expression chips to reveal altered expression of genes encoding secreted proteins. Keywords: Growth factor stimulation experiment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4865

2 Samples

Download data: TXT

(Submitter supplied) Mouse embryonic fibroblasts stimulated with 40 ng/ml of FGF2 in conventional hES cell medium were analysed on whole-genome expression chips to reveal altered expression of genes encoding secreted proteins. Keywords: Growth factor stimulation experiment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4865

2 Samples

Download data: TXT

(Submitter supplied) Hybridisations were carried out to define a fibroblast-like cell type derived from H9 human embryonic stem cells. Keywords: cell type comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2423

Platform:

GPL2700

4 Samples

Download data: TXT

(Submitter supplied) This series includes hES cell samples starved and re-stimulated with FGF2. Controls were FGF2-starved but not re-stimulated. Two independent experiments were carried out (one sample plus control each). Keywords: Growth factor stimulation experiment

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2422

Platform:

GPL2700

4 Samples

Download data: TXT

Analysis of embryonic fibroblasts starved of fibroblast growth factor 2 (FGF2) for 2 days and subsequently restimulated with FGF2. FGF2 promotes the self-renewal of embryonic stem cells (ESCs) . Results provide insight into the molecular mechanisms underlying ESC self-renewal in response to FGF2.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:

GPL2700

Series:

GSE5415

4 Samples

Download data: TXT

Analysis of embryonic fibroblasts after treatment with fibroblast growth factor 2 (FGF2) at 4, 13, and 40 ng/ml for 24 hours. FGF2 promotes the self-renewal of embryonic stem cells (ESCs) . Results provide insight into the molecular mechanisms underlying ESC self-renewal in response to FGF2.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 dose sets

Platform:

GPL4055

Series:

GSE5437

4 Samples

Download data: TXT

(Submitter supplied) Directing differentiation of human embryonic stem cells (hESC) into specific cell types using an easy and reproducible protocol is a perquisite for the clinical use of hESC in regenerative medicine protocols. Here, we report the generation of mesodermal cells with differentiation potential to myocytes, osteoblasts, chondrocytes and adipocytes. We demonstrate that during hESC differentiation as embryoid bodies (EB), inhibition of TGF-b/Activin/Nodal signaling using SB-431542 (SB) markedly up-regulated paraxial mesodermal markers (TBX6, TBX5), early myogenic transcriptional factors (Myf5, Pax7) as well as myocyte committed markers (NCAM, CD34, Desmin, MHC (fast), alpha-smooth muscle actin, Nkx2.5, cTNT). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL, CHP

(Submitter supplied) A fully defined media for human embryonic stem cells (hESCs) was designed after examining the phosphorylation status of 42 receptor tyrosine kinases (RTKs) on hESCs exposed to mouse embryonic fibroblast conditioned medium (CM). Activation of the insulin and insulin-like growth factor-1 receptors (IR, IGF1R) as well as EGFR family members including ERBB2 and ERBB3 led to the design of a simple defined media which supported robust long-term growth of multiple hESC lines and allowed massive expansion of undifferentiated cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2507

4 Samples

Download data: TXT

(Submitter supplied) It has been demonstrated that CHIR99021 promotes self-renewal of mouse embryonic stem cells, however, the target genes of CHIR99021 is not fully understood. AICAR is the activator of AMP-activated protein kinase. It is reported that AICAR plays important role in mouse embryonic stem cells, however the moleculor mechanism of this phenomenon is unknown. To better understand the downstream target genes of CHIR99021 and AICAR, we performed Microarray analyses to identify their downstream targets. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

9 Samples

Download data: TXT

(Submitter supplied) Recent studies suggest that telomerase promotes cell growth by mechanisms that extend beyond the rescue of critically short telomeres. The in vitro model of mTert overexpressing MEFs recapitulates fundamental aspects of the growth-promoting effects of mTert in vivo. First, in Terc-proficient cells, mTert overexpression favors escape from replicative senescence and enhances anchorage-independent growth in response to oncogenic stress, which fits well with previous data showing that mTert overexpression promotes tumor formation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1211

Platform:

GPL339

4 Samples

Download data: CEL, CHP, EXP, RPT, TXT

Expression profiling of embryonic fibroblasts overexpressing mTert, the catalytic subunit of telomerase. Results identify potential downstream targets of telomerase.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL339

Series:

GSE2684

4 Samples

Download data: CEL, CHP, EXP, RPT, TXT

(Submitter supplied) Bone morphogenetic protein (BMP) signaling is known to support differentiation of human embryonic stem cells (hESCs) into mesoderm and extraembryonic lineages, whereas other signaling pathways can largely influence this lineage specification. Here, we set out to reinvestigate the influence of ACTIVIN/NODAL and fibroblast growth factor (FGF) pathways on the lineage choices made by hESCs during BMP4-driven differentiation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

24 Samples

Download data: TXT

(Submitter supplied) H1 hESC were exposed to BMP4 (10 ng/ml) in absence of FGF2 for 0, 3, 12, 24, 72, and 120 h under both physiological (4 % O2) and atmospheric (20 % O2) conditions to identify candidate genes and gene networks associated with specification from totipotent precursor cells to trophoblast. RNA from cells under both conditions was isolated at each time point. Approximately 32,000 probe sets (out of 40,391) provided a signal above background at one of the time points. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

20 Samples

Download data: TXT

(Submitter supplied) This experiment records the transcriptional responses of mES cells (line OG2) to FGF/ERK stimulation in the presence of LIF, to LIF/STAT3 inhibition in the presence of an FGF/ERK inhibitor, and to combined FGF/ERK stimulation / LIF/STAT3 inhibition.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

4 Samples

Download data: TXT

(Submitter supplied) This experiment records the transcriptional responses of EpiSCs (line E3) to FGF/ERK inhibition, GSK3ß inhibition, LIF/STAT3 activation, as well as combined treatment for 12 hours each.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

5 Samples

Download data: TXT

(Submitter supplied) This experiment records the transcriptional responses of EpiSCs (line E3) to FGF/ERK inhibition, GSK3ß inhibition, LIF/STAT3 activation, as well as combined treatment for 3 hours each.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

5 Samples

Download data: TXT

(Submitter supplied) This analysis compares the expression profiles of CGR8 ES cells, E3 epiblast stem cells, and E3R cells (E3 EpiSCs reverted to an ES-like state).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

3 Samples

Download data: TXT