GEO DataSets

(Submitter supplied) In this study we combined small RNA isolation from post-ribosomal supernatant with RibOxi-seq to identify 2’-O-methylation (Nm) in single nucleotide resolution. Our analysis identified ~49 Nm sites in tRNA, snRNAs, snoRNAs, 7SL RNA and vtRNA.

Organism:

Trypanosoma brucei brucei TREU927

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL30921

4 Samples

Download data: CSV

(Submitter supplied) Under persistent ER stress, Trypanosoma brucei parasites induce the spliced leader silencing (SLS) pathway. In SLS, transcription of the SL RNA gene, the SL donor to all mRNAs, is extinguished, arresting transsplicing and leading to programmed cell death(PCD). In this study, we investigated the transcriptome following silencing of SEC63, a factor essential for protein translocation across the ER membrane, and whose silencing induces SLS. more...

Organism:

Trypanosoma brucei; Trypanosoma brucei brucei TREU927

Type:

Expression profiling by array

Platforms:

GPL22323 GPL22322

8 Samples

Download data: GPR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Trypanosoma brucei; Trypanosoma brucei brucei TREU927

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL17034 GPL17033

24 Samples

Download data: PAIR, TXT

(Submitter supplied) Three potential ELAV-like proteins of T. brucei, including Tb927.3.2930, Tb927.7.5380, and Tb927.8.6650, were either inhibited by RNAi or phenotypically activated by over-expression, followed by microarray analysis of the transcriptome. The results indicated that these ELAV-like proteins regulate the abundance of a large number of T. brucei transcripts, potentially through regulation of mRNA stability.

Organism:

Trypanosoma brucei; Trypanosoma brucei brucei TREU927

Type:

Expression profiling by array

Platform:

GPL17034

22 Samples

Download data: PAIR

(Submitter supplied) Whole genome comparison of RNA levels for both protein coding genes and structural RNAs in five different life cycle stages: in vivo slender bloodstream form, in vivo stumpy bloodstream form, cultured bloodstream form, log-phase procyclic culture form and stationary-phase procyclic culture form

Organism:

Trypanosoma brucei brucei TREU927

Type:

Expression profiling by genome tiling array

Platform:

GPL9285

15 Samples

Download data: TXT

(Submitter supplied) This set includes 8381 oligonucleotides, has 37632 spots distributed in 48 blocks. Protocol: 2QTB, with arrayer GENETIX, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000

Organism:

Trypanosoma brucei brucei TREU927

1 Series

4 Samples

Download data

(Submitter supplied) This set includes 8375 oligonucleotides, has 19200 spots distributed in 48 blocks. Protocol: 3QTB, with arrayer GENETIX, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000

Organism:

Trypanosoma brucei brucei TREU927

1 Series

4 Samples

Download data

(Submitter supplied) DESIGN_NAME: 120828_Tbrucei927_RS_exp DESIGN_ID: 574071 The Platform data table reflects a condensed representation of the array's replicate features. The full array layout representing all of the individual features (and probe sequences) is linked as a supplementary file at the foot of this record. Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Trypanosoma brucei brucei TREU927

2 Series

22 Samples

Download data: NDF, NGD

(Submitter supplied) 60mer probes were designed to known coding regions of Trypanosoma brucei strain 927, Approximately 8 probes per gene were designed to each of the 8562 protein coding genes, and 3 probes were designed to each RNA gene. Each probe was placed on the chip in triplicate. Remaining space on the array was fillied with 50mer probes spaced evenly through unnannotated regions of the genome, though these probes were not considered in the final analysis. more...

Organism:

Trypanosoma brucei brucei TREU927

1 Series

15 Samples

Download data: NDF, NGD, TXT